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TGFBI Recombinant Rabbit Monoclonal Antibody [PSH28-78]

Usd: 385

Specification

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Catalog# HA724809

TGFBI Recombinant Rabbit Monoclonal Antibody [PSH28-78]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

TGFBI Recombinant Rabbit Monoclonal Antibody [PSH28-78]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human TGFBI aa 1-683.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P

Target Molecular Weight

Predicted band size: 75 kDa

Positive Control

A549 (Human lung adenocarcinoma cell) cell lysate, A549 treated with 5ng/mL hTGFβ1 for 48 hours add 300ng/mL BFA for last 16 hours cell lysate, NIH:OVCAR-3 (Human ovarian adenocarcinoma cell) cell lysates.

Conjugation

unconjugated

Clone Number

PSH28-78

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P FC(Intra) IF-Cell
human
Tested Tested
Tested Tested
Not Recommended
Tested Tested

Product Features

Form

Liquid

Concentration

0.5 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:200

  • IHC-P

  • 1:2,000-1:10,000

Target

Function

Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene, locus 5q31. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.

Background References

1. Huang AH et al. Evaluation of the complex transcriptional topography of mesenchymal stem cell chondrogenesis for cartilage tissue engineering. Tissue Eng Part A 16:2699-708 (2010).

2. He Y et al. Largescale Transcriptomics Analysis Suggests Over-Expression of BGH3, MMP9 and PDIA3 in Oral Squamous Cell Carcinoma. PLoS One 11:e0146530 (2016).

Subcellular Location

Secreted, extracellular space, extracellular matrix.

UNIPROT

SwissProt: Q15582 Human

Synonyms

RGD containing collagen associated protein antibody

AI181842 antibody

AI747162 antibody

Beta ig antibody

Beta ig h3 antibody

Beta ig-h3 antibody

BGH3_HUMAN antibody

Big h3 antibody

BIGH3 antibody

CDB1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of TGFBI on different lysates with Rabbit anti-TGFBI antibody (<a href="/products/HA724809" style="font-weight: bold;text-decoration: underline;">HA724809</a>) at 1/2,000 dilution. <br /> <br />Lane 1: A549 (Human lung adenocarcinoma cell) cell lysate <br />Lane 2: A549 treated with 5ng/mL hTGFβ1 for 48 hours add 300ng/mL BFA for last 16 hours cell lysate <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 10 seconds; ECL: K1801 <br /> <br />The molecule weight observed is consistent with what has been described in the literature (PMID: 39068456, PMID: 37865162). <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724809" style="font-weight: bold;text-decoration: underline;">HA724809</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 75 kDa <br />Observed band size: 70 kDa

    ☑ Cell treatment (CT)

    Western blot analysis of TGFBI on different lysates with Rabbit anti-TGFBI antibody (HA724809) at 1/2,000 dilution.

    Lane 1: A549 (Human lung adenocarcinoma cell) cell lysate
    Lane 2: A549 treated with 5ng/mL hTGFβ1 for 48 hours add 300ng/mL BFA for last 16 hours cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    The molecule weight observed is consistent with what has been described in the literature (PMID: 39068456, PMID: 37865162).

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724809, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 75 kDa
    Observed band size: 70 kDa

  • Western blot analysis of TGFBI on NIH:OVCAR-3 (Human ovarian adenocarcinoma cell) cell lysate with Rabbit anti-TGFBI antibody (<a href="/products/HA724809" style="font-weight: bold;text-decoration: underline;">HA724809</a>) at 1/2,000 dilution. <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 10 seconds; ECL: K1801 <br /> <br />The molecule weight observed is consistent with what has been described in the literature (PMID: 39068456, PMID: 37865162). <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724809" style="font-weight: bold;text-decoration: underline;">HA724809</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 75 kDa <br />Observed band size: 70 kDa

    Western blot analysis of TGFBI on NIH:OVCAR-3 (Human ovarian adenocarcinoma cell) cell lysate with Rabbit anti-TGFBI antibody (HA724809) at 1/2,000 dilution.

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    The molecule weight observed is consistent with what has been described in the literature (PMID: 39068456, PMID: 37865162).

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724809, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 75 kDa
    Observed band size: 70 kDa

  • Application: Immunocytochemistry (IF-cell) <br /> <br />Species: Human <br />Sample: A549 (Human lung adenocarcinoma cell) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. <br />Antibody dilution buffer: 1% BSA in PBST. <br /> <br />Primary antibody: <a href="/products/HA724809" style="font-weight: bold;text-decoration: underline;">HA724809</a>, 1/200, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 45 minutes at room temperature. <br />Counterstain: Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

    Application: Immunocytochemistry (IF-cell)

    Species: Human
    Sample: A549 (Human lung adenocarcinoma cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.

    Primary antibody: HA724809, 1/200, overnight at 4°C.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
    Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Squamous cell carcinoma of the lung <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724809" style="font-weight: bold;text-decoration: underline;">HA724809</a>, 1/8,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Squamous cell carcinoma of the lung
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724809, 1/8,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Colon <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724809" style="font-weight: bold;text-decoration: underline;">HA724809</a>, 1/2,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Colon
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724809, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Kidney <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724809" style="font-weight: bold;text-decoration: underline;">HA724809</a>, 1/2,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Kidney
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724809, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

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