Cleaved+pro Caspase-3 Recombinant Rabbit Monoclonal Antibody [PSH29-36]
Catalog# HA724865
Cleaved+pro Caspase-3 Recombinant Rabbit Monoclonal Antibody [PSH29-36]
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WB
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IHC-P
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FC(Intra)
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Cleaved+pro Caspase-3 Recombinant Rabbit Monoclonal Antibody [PSH29-36]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within rat Caspase-3 aa 1-277.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, FC(Intra)
Target Molecular Weight
Predicted band size: 32 kDa
Positive Control
HeLa (Human cervical adenocarcinoma cell) cell lysate, HeLa treated with 1μM staurosporine for 3 hours cell lysate, Jurkat (Human T-lymphoblastic cells) cell lysate, Jurkat treated with 1μM staurosporine for 3 hours cell lysate, C6 (Rat glioma cell) cell lysate, C6 treated with 1μM staurosporine for 3 hours cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, NIH/3T3 treated with 1μM staurosporine for 3 hours cell lysate, C2C12 (Mouse myoblast) cell lysate, C2C12 treated with 1μM staurosporine for 3 hours cell lysate.
Conjugation
unconjugated
Clone Number
PSH29-36
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC(Intra) | IF-Cell | |
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| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
0.5 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
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IHC-P
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1:10,000
-
FC(Intra)
-
1:500
Target
Function
Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9. It is encoded by the CASP3 gene. CASP3 orthologs have been identified in numerous mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts. Caspase-3 shares many of the typical characteristics common to all currently-known caspases. For example, its active site contains a cysteine residue (Cys-163) and histidine residue (His-121) that stabilize the peptide bond cleavage of a protein sequence to the carboxy-terminal side of an aspartic acid when it is part of a particular 4-amino acid sequence. This specificity allows caspases to be incredibly selective, with a 20,000-fold preference for aspartic acid over glutamic acid. A key feature of caspases in the cell is that they are present as zymogens, termed procaspases, which are inactive until a biochemical change causes their activation. Each procaspase has an N-terminal large subunit of about 20 kDa followed by a smaller subunit of about 10 kDa, called p20 and p10, respectively.
Background References
1. Dong L et al. Echinacoside Induces Apoptosis in Human SW480 Colorectal Cancer Cells by Induction of Oxidative DNA Damages. Int J Mol Sci 16:14655-68 (2015).
2. Nilsonne G et al. Phenotype-dependent apoptosis signalling in mesothelioma cells after selenite exposure. J Exp Clin Cancer Res 28:92 (2009).
Subcellular Location
Cytoplasm.
Synonyms
Caspase3
A830040C14Rik antibody
Apopain antibody
CASP 3 antibody
CASP-3 antibody
CASP3 antibody
CASP3_HUMAN antibody
Casp3a antibody
Caspase 3 antibody
Caspase 3, apoptosis-related cysteine peptidase antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Cleaved+pro Caspase-3 on different lysates with Rabbit anti-Cleaved+pro Caspase-3 antibody (HA724865) at 1/2,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: HeLa treated with 1μM staurosporine for 3 hours cell lysate
Lane 3: Jurkat (Human T-lymphoblastic cells) cell lysate
Lane 4: Jurkat treated with 1μM staurosporine for 3 hours cell lysate
Lane 5: C6 (Rat glioma cell) cell lysate
Lane 6: C6 treated with 1μM staurosporine for 3 hours cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724865, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 32 kDa
Observed band size: 32/17 kDa -
☑ Cell treatment (CT)
Western blot analysis of Cleaved+pro Caspase-3 on different lysates with Rabbit anti-Cleaved+pro Caspase-3 antibody (HA724865) at 1/2,000 dilution.
Lane 1: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 2: NIH/3T3 treated with 1μM staurosporine for 3 hours cell lysate
Lane 3: C2C12 (Mouse myoblast) cell lysate
Lane 4: C2C12 treated with 1μM staurosporine for 3 hours cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 25 seconds ; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724865, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 32 kDa
Observed band size: 32/17 kDa -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724865, 1/10,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Lymph node
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724865, 1/10,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724865, 1/10,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Lymph node
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724865, 1/10,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Flow Cytometry (Intra)
Species: Rat
Sample: C6 (Rat glioma cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724865 (1/500, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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