Human CXCL10 / IP-10 Recombinant Rabbit Monoclonal Antibody [PSH12-36] - BSA and Azide free (Capture)
Overview
Product Name
Human CXCL10 / IP-10 Recombinant Rabbit Monoclonal Antibody [PSH12-36] - BSA and Azide free (Capture)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human CXCL10 aa 22-98 (HA210896).
Species Reactivity
Human, Cynomolgus monkey
Validated Applications
ELISA(Cap)
Positive Control
Recombinant Human CXCL10 protein (HA210896).
Conjugation
unconjugated
Clone Number
PSH12-36
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-37] to Human CXCL10 / IP-10 antibody (Detector) (HA725008) and Recombinant Human CXCL10 / IP-10 protein (HA210896) as the standard. The reference range value is 1.6-200 pg/ml.
Target
Function
C-X-C motif chemokine ligand 10 (CXCL10) also known as Interferon gamma-induced protein 10 (IP-10) or small-inducible cytokine B10 is an 8.7 kDa protein that in humans is encoded by the CXCL10 gene. C-X-C motif chemokine 10 is a small cytokine belonging to the CXC chemokine family. CXCL10 is secreted by several cell types in response to IFN-γ. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.
Background References
1. Limagne E et al. MEK inhibition overcomes chemoimmunotherapy resistance by inducing CXCL10 in cancer cells. Cancer Cell. 2022 Feb
2. Li X et al. CXCL10-armed oncolytic adenovirus promotes tumor-infiltrating T-cell chemotaxis to enhance anti-PD-1 therapy. Oncoimmunology. 2022 Aug
Subcellular Location
Secreted.
UNIPROT
Synonyms
Interferon gamma induced factor MOB1, mouse, homolog of antibody
Interferon gamma induced protein 10 antibody
10 kDa interferon gamma induced protein antibody
10 kDa interferon gamma-induced protein antibody
C X C motif chemokine 10 antibody
C7 antibody
Chemokine (C X C motif) ligand 10 antibody
Chemokine CXC motif ligand 10 antibody
Crg 2 antibody
CRG2 antibody
ExpandImages
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![Sandwich ELISA analysis of human CXCL10 matched pair antibodies<br /><br />Capture: <a href="/products/HA725007" style="font-weight: bold;text-decoration: underline;">HA725007</a>, Human CXCL10 / IP-10 Rabbit mAb [PSH12-36]<br />Detector: <a href="/products/HA725008" style="font-weight: bold;text-decoration: underline;">HA725008</a>, Human CXCL10 / IP-10 Rabbit mAb [PSH12-37]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725007" style="font-weight: bold;text-decoration: underline;">HA725007</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CXCL10 protein (<a href="/products/HA210896" style="font-weight: bold;text-decoration: underline;">HA210896</a>) starting from 200 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725008" style="font-weight: bold;text-decoration: underline;">HA725008</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725007_1.png?v=20260401003021)
Sandwich ELISA analysis of human CXCL10 matched pair antibodies
Capture: HA725007, Human CXCL10 / IP-10 Rabbit mAb [PSH12-36]
Detector: HA725008, Human CXCL10 / IP-10 Rabbit mAb [PSH12-37]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725007) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CXCL10 protein (HA210896) starting from 200 pg/ml to 0 pg/ml and detect antibody (HA725008, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native CXCL10 in THP-1 supernatant samples.<br /><br />Capture: <a href="/products/HA725007" style="font-weight: bold;text-decoration: underline;">HA725007</a>, Human CXCL10 / IP-10 Rabbit mAb [PSH12-36]<br />Detector: <a href="/products/HA725008" style="font-weight: bold;text-decoration: underline;">HA725008</a>, Human CXCL10 / IP-10 Rabbit mAb [PSH12-37]<br /><br />Interpolated concentration of native CXCL10 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CXCL10 concentration was determined to be 2993 pg/mL in THP-1+hu IFN-γ (1μg/ml 8h) then LPS (1μg/mL overnight) sups (+) sups, 7 pg/ml in THP-1+hu IFN-γ (1μg/ml 8h) then LPS (1μg/mL overnight) sups (-) cell culture supernatant.](https://storage.huabio.cn/huabio/productImg/HA725007_2.png?v=20260401003021)
Interpolated concentrations of native CXCL10 in THP-1 supernatant samples.
Capture: HA725007, Human CXCL10 / IP-10 Rabbit mAb [PSH12-36]
Detector: HA725008, Human CXCL10 / IP-10 Rabbit mAb [PSH12-37]
Interpolated concentration of native CXCL10 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CXCL10 concentration was determined to be 2993 pg/mL in THP-1+hu IFN-γ (1μg/ml 8h) then LPS (1μg/mL overnight) sups (+) sups, 7 pg/ml in THP-1+hu IFN-γ (1μg/ml 8h) then LPS (1μg/mL overnight) sups (-) cell culture supernatant.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Biotin Conjugated Human CXCL10 / IP-10 Recombinant Rabbit Monoclonal Antibody [PSH12-37] - Detector
Application: ELISA(Det),ELISA
Reactivity: Human,Cynomolgus monkey
Conjugate: Biotin
