Biotin Conjugated Human IL-25 Recombinant Rabbit Monoclonal Antibody [PSH15-24] - Detector
Overview
Product Name
Biotin Conjugated Human IL-25 Recombinant Rabbit Monoclonal Antibody [PSH15-24] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human IL-25 aa 33-177 (HA211098).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human IL-25 protein (HA211098).
Conjugation
Biotin
Clone Number
PSH15-24
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH15-23] to Human IL-25 antibody (Capture) (HA725016) and Recombinant Human IL-25 protein (HA211098) as the standard. The reference range value is 7.8-1,000 pg/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
Cytokine produced by various cells such as eosinophils, T-helper type 2 (Th2) cells or epithelial cells that plays a role in internal safety of adaptive immune responses by regulating cytokine production. Promotes and augments T-helper type 2 responses locally or systemically. Exerts its activity via its receptor composed of IL17RA and IL17RB for signal transduction (By similarity). In turn, stimulates the JAK2-STAT5A pathway and promotes the secretion of type-2 associated cytokines including IL4, IL9 and IL13. Induces also the release of IL8, and IL6 from eosinophils through the combined activation of MAPK and NF-kappa-B pathways. Inhibits the differentiation of T-helper (Th17) cells via the production of IL4, IL5 and IL13.
Background References
1. Liu J et al. IL25 Enhanced Colitis-Associated Tumorigenesis in Mice by Upregulating Transcription Factor GLI1. Front Immunol. 2022 Mar
2. Ozaki Y et al. IL25(+) macrophages are a key determinant of treatment resistance of IL17RB(+) breast cancer. Am J Cancer Res. 2023 Oct
Subcellular Location
Secreted.
UNIPROT
Synonyms
IL 17E antibody
IL 25 antibody
IL-17E antibody
IL-25 antibody
IL17e antibody
IL25 antibody
IL25_HUMAN antibody
Interleukin 17E antibody
Interleukin 25 antibody
Interleukin-17E antibody
ExpandImages
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![Sandwich ELISA analysis of Human IL-25 matched pair antibodies<br /><br />Capture: <a href="/products/HA725016" style="font-weight: bold;text-decoration: underline;">HA725016</a>, Human IL-25 Rabbit mAb [PSH15-23]<br />Detector: <a href="/products/HA725017" style="font-weight: bold;text-decoration: underline;">HA725017</a>, Human IL-25 Rabbit mAb [PSH15-24]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725016" style="font-weight: bold;text-decoration: underline;">HA725016</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-25 protein (<a href="/products/HA211098" style="font-weight: bold;text-decoration: underline;">HA211098</a>) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725017" style="font-weight: bold;text-decoration: underline;">HA725017</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725018B_1.jpg?v=20260604174757)
Sandwich ELISA analysis of Human IL-25 matched pair antibodies
Capture: HA725016, Human IL-25 Rabbit mAb [PSH15-23]
Detector: HA725017, Human IL-25 Rabbit mAb [PSH15-24]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725016) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-25 protein (HA211098) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA725017, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"