Biotin Conjugated Mouse GM-CSF Recombinant Rabbit Monoclonal Antibody [PSH12-41] - Detector
Overview
Product Name
Biotin Conjugated Mouse GM-CSF Recombinant Rabbit Monoclonal Antibody [PSH12-41] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse GM-CSF aa 18-141 (HA210930).
Species Reactivity
Mouse
Validated Applications
ELISA(Det), ELISA
Molecular Weight
Predicted band size: 16 kDa
Positive Control
Recombinant Mouse GM-CSF protein (HA210930).
Conjugation
Biotin
Clone Number
PSH12-41
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-40] to Mouse GM-CSF antibody (Capture) (HA725019) and Recombinant Mouse GM-CSF protein (HA210930) as the standard. The reference range value is 15.6-2,000 pg/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim. Unlike granulocyte colony-stimulating factor, which specifically promotes neutrophil proliferation and maturation, GM-CSF affects more cell types, especially macrophages and eosinophils.
Background References
1. Kumar A et al. GM-CSF: A Double-Edged Sword in Cancer Immunotherapy. Front Immunol. 2022 Jul
2. Chen Y et al. Role of GM-CSF in lung balance and disease. Front Immunol. 2023 Apr
Subcellular Location
Secreted.
UNIPROT
Synonyms
Colony stimulating factor 2 (granulocyte-macrophage) antibody
Colony Stimulating Factor 2 antibody
Colony stimulating factor antibody
Colony-stimulating factor antibody
CSF 2 antibody
CSF antibody
CSF2 antibody
CSF2_HUMAN antibody
GM-CSF antibody
GMCSF antibody
ExpandImages
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![Sandwich ELISA analysis of 0 Mouse GM-CSF matched pair antibodies<br /><br />Capture: <a href="/products/HA725019" style="font-weight: bold;text-decoration: underline;">HA725019</a>, Mouse GM-CSF Rabbit mAb [PSH12-40]<br />Detector: <a href="/products/HA725020" style="font-weight: bold;text-decoration: underline;">HA725020</a>, Mouse GM-CSF Rabbit mAb [PSH12-41]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725019" style="font-weight: bold;text-decoration: underline;">HA725019</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse GM-CSF protein (<a href="/products/HA210930" style="font-weight: bold;text-decoration: underline;">HA210930</a>) starting from 2000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725020" style="font-weight: bold;text-decoration: underline;">HA725020</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725021B_1.png?v=20260604174822)
Sandwich ELISA analysis of 0 Mouse GM-CSF matched pair antibodies
Capture: HA725019, Mouse GM-CSF Rabbit mAb [PSH12-40]
Detector: HA725020, Mouse GM-CSF Rabbit mAb [PSH12-41]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725019) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse GM-CSF protein (HA210930) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA725020, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native GM-CSF in RAW264.7 supernatant treated or untreated with LPS for 48h.<br /><br />Capture: <a href="/products/HA725019" style="font-weight: bold;text-decoration: underline;">HA725019</a>, Mouse GM-CSF Rabbit mAb [PSH12-40]<br />Detector: <a href="/products/HA725020" style="font-weight: bold;text-decoration: underline;">HA725020</a>, Mouse GM-CSF Rabbit mAb [PSH12-41]<br /><br />Interpolated concentration of native GM-CSF was measured in duplicate at different sample concentrations and interpolated from the GM-CSF standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean GM-CSF concentration was determined to be 17,161 pg/mL in neat RAW264.7 treated supernatant and 126 pg/mL in untreated RAW264.7 supernatant.](https://storage.huabio.cn/huabio/productImg/HA725021B_2.png?v=20260604174822)
Interpolated concentrations of native GM-CSF in RAW264.7 supernatant treated or untreated with LPS for 48h.
Capture: HA725019, Mouse GM-CSF Rabbit mAb [PSH12-40]
Detector: HA725020, Mouse GM-CSF Rabbit mAb [PSH12-41]
Interpolated concentration of native GM-CSF was measured in duplicate at different sample concentrations and interpolated from the GM-CSF standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean GM-CSF concentration was determined to be 17,161 pg/mL in neat RAW264.7 treated supernatant and 126 pg/mL in untreated RAW264.7 supernatant.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"