Biotin Conjugated Mouse CXCL9 Recombinant Rabbit Monoclonal Antibody [PSH12-63]
Overview
Product Name
Biotin Conjugated Mouse CXCL9 Recombinant Rabbit Monoclonal Antibody [PSH12-63]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mosue CXCL9 aa 22-126 (HA211031).
Species Reactivity
Mouse
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Mouse CXCL9 protein (HA211031).
Conjugation
Biotin
Clone Number
PSH12-63
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-62] to Mouse CXCL9 antibody (Capture) (HA725022) and Recombinant Mouse CXCL9 protein (HA211013) as the standard. The reference range value is 15.6-2,000 pg/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
This antimicrobial gene is part of a chemokine superfamily that encodes secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded is thought to be involved in T cell trafficking. The encoded protein binds to C-X-C motif chemokine 3 and is a chemoattractant for lymphocytes but not for neutrophils. Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.
Background References
1. Kersh A.E., Sati S., Huang J., Murphy C., Ahart O., Leung T.H. CXCL9, CXCL10, and CCL19 synergistically recruit T lymphocytes to skin in lichen planus. JCI Insight 9:e179899-e179899 (2024)
2. Larkin R.M., Lopez D.C., Robbins Y.L., Lassoued W., Canubas K., Warner A., Karim B., Vulikh K., Hodge J.W., London N.R. Jr. Augmentation of tumor expression of HLA-DR, CXCL9, and CXCL10 may improve olfactory neuroblastoma immunotherapeutic responses. J Transl Med 22:524-524 (2024)
Subcellular Location
Secreted.
UNIPROT
Synonyms
C-X-C motif chemokine 9 antibody
chemokine (C-X-C motif) ligand 9 antibody
CMK antibody
crg-10 antibody
CXCL9 antibody
CXCL9_HUMAN antibody
gamma interferon induced monokine antibody
Gamma-interferon-induced monokine antibody
HuMIG antibody
MIG antibody
ExpandImages
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![Sandwich ELISA analysis of mouse CXCL9 matched pair antibodies<br /><br />Capture: <a href="/products/HA725022" style="font-weight: bold;text-decoration: underline;">HA725022</a>, Mouse CXCL9 Rabbit mAb [PSH12-62]<br />Detector: <a href="/products/HA725023" style="font-weight: bold;text-decoration: underline;">HA725023</a>, Mouse CXCL9 Rabbit mAb [PSH12-63]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA725022" style="font-weight: bold;text-decoration: underline;">HA725022</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CXCL9 protein (<a href="/products/HA211031" style="font-weight: bold;text-decoration: underline;">HA211031</a>) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725023" style="font-weight: bold;text-decoration: underline;">HA725023</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725024B_1.png?v=20260604174820)
Sandwich ELISA analysis of mouse CXCL9 matched pair antibodies
Capture: HA725022, Mouse CXCL9 Rabbit mAb [PSH12-62]
Detector: HA725023, Mouse CXCL9 Rabbit mAb [PSH12-63]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725022) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CXCL9 protein (HA211031) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725023, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native CXCL9 in unstimulated and stimulated J774A.1 cell culture supernatant.<br /><br />Capture: <a href="/products/HA725022" style="font-weight: bold;text-decoration: underline;">HA725022</a>, Mouse CXCL9 Rabbit mAb [PSH12-62]<br />Detector: <a href="/products/HA725023" style="font-weight: bold;text-decoration: underline;">HA725023</a>, Mouse CXCL9 Rabbit mAb [PSH12-63]<br /><br />J774A.1 cells were stimulated with 20 ng/ml recombinant mouse IFN-γ and 1 μg/ml LPS incubated for 1 day. The concentrations of CXCL9 measured in duplicate and interpolated from the CXCL9 standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 1.6%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL9 concentration was determined to be 17,738 pg/ml in IFN-γ and LPS stimulated J774A.1 cell culture supernatant and undetectable in the unstimulated J774A.1 control.](https://storage.huabio.cn/huabio/productImg/HA725024B_2.png?v=20260604174820)
Interpolated concentrations of native CXCL9 in unstimulated and stimulated J774A.1 cell culture supernatant.
Capture: HA725022, Mouse CXCL9 Rabbit mAb [PSH12-62]
Detector: HA725023, Mouse CXCL9 Rabbit mAb [PSH12-63]
J774A.1 cells were stimulated with 20 ng/ml recombinant mouse IFN-γ and 1 μg/ml LPS incubated for 1 day. The concentrations of CXCL9 measured in duplicate and interpolated from the CXCL9 standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 1.6%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL9 concentration was determined to be 17,738 pg/ml in IFN-γ and LPS stimulated J774A.1 cell culture supernatant and undetectable in the unstimulated J774A.1 control.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"