Mouse/Rat CXCL1/GRO alpha Recombinant Rabbit Monoclonal Antibody [PSH13-15] - BSA and Azide free (Detector)
Overview
Product Name
Mouse/Rat CXCL1/GRO alpha Recombinant Rabbit Monoclonal Antibody [PSH13-15] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse CXCL1 aa 25-96 (HA210792).
Species Reactivity
Mouse, Rat
Validated Applications
ELISA(Det)
Positive Control
Recombinant Mouse CXCL1 protein (HA210792) or Rat CXCL1 protein (HA211010).
Conjugation
unconjugated
Clone Number
PSH13-15
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-14] to Mouse/Rat CXCL1/GRO alpha antibody (Capture) (HA725028) and recombinant Mouse CXCL1 protein (HA210792) or recombinant Rat CXCL1 protein (HA211010) as the standard. The reference range value is 7.8-1,000 pg/mL.
Target
Function
The chemokine (C-X-C motif) ligand 1 (CXCL1) is a small peptide belonging to the CXC chemokine family that acts as a chemoattractant for several immune cells, especially neutrophils or other non-hematopoietic cells to the site of injury or infection and plays an important role in regulation of immune and inflammatory responses. It was previously called GRO1 oncogene, GROα, neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MGSA-α). CXCL1 was first cloned from a cDNA library of genes induced by platelet-derived growth factor (PDGF) stimulation of BALB/c-3T3 murine embryonic fibroblasts and named "KC" for its location in the nitrocellulose colony hybridization assay. This designation is sometimes erroneously believed to be an acronym and defined as "keratinocytes-derived chemokine". Rat CXCL1 was first reported when NRK-52E (normal rat kidney-52E) cells were stimulated with interleukin-1β (IL-1β) and lipopolysaccharide (LPS) to generate a cytokine that was chemotactic for rat neutrophils, cytokine-induced neutrophil chemoattractant (CINC). In humans, this protein is encoded by the gene CXCL1 and is located on human chromosome 4 among genes for other CXC chemokines.
Background References
1. Korbecki J et al. CXCL1: Gene, Promoter, Regulation of Expression, mRNA Stability, Regulation of Activity in the Intercellular Space. Int J Mol Sci. 2022 Jan
2. Chen H et al. METTL3 Inhibits Antitumor Immunity by Targeting m(6)A-BHLHE41-CXCL1/CXCR2 Axis to Promote Colorectal Cancer. Gastroenterology. 2022 Oct
Subcellular Location
Secreted.
Synonyms
C-X-C motif chemokine 1 antibody
Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) antibody
chemokine (C-X-C motif) ligand 1 antibody
CINC-1 antibody
CXCL1 antibody
Cytokine-induced neutrophil chemoattractant 1 antibody
Fibroblast secretory protein antibody
Fsp antibody
Gro 1 antibody
Gro A antibody
ExpandImages
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![Sandwich ELISA analysis of Mouse CXCL1 matched pair antibodies<br /><br />Capture: <a href="/products/HA725028" style="font-weight: bold;text-decoration: underline;">HA725028</a>, Mouse/Rat CXCL1/GRO Rabbit mAb [PSH13-14]<br />Detector: <a href="/products/HA725029" style="font-weight: bold;text-decoration: underline;">HA725029</a>, Mouse/Rat CXCL1/GRO Rabbit mAb [PSH13-15]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725028" style="font-weight: bold;text-decoration: underline;">HA725028</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CXCL1 protein (<a href="/products/HA210792" style="font-weight: bold;text-decoration: underline;">HA210792</a>) starting from 1000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725029" style="font-weight: bold;text-decoration: underline;">HA725029</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725029_1.png?v=20260401003030)
Sandwich ELISA analysis of Mouse CXCL1 matched pair antibodies
Capture: HA725028, Mouse/Rat CXCL1/GRO Rabbit mAb [PSH13-14]
Detector: HA725029, Mouse/Rat CXCL1/GRO Rabbit mAb [PSH13-15]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725028) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CXCL1 protein (HA210792) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA725029, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Sandwich ELISA analysis of Rat CXCL1 matched pair antibodies<br /><br />Capture: <a href="/products/HA725028" style="font-weight: bold;text-decoration: underline;">HA725028</a>, Mouse/Rat CXCL1/GRO Rabbit mAb [PSH13-14]<br />Detector: <a href="/products/HA725029" style="font-weight: bold;text-decoration: underline;">HA725029</a>, Mouse/Rat CXCL1/GRO Rabbit mAb [PSH13-15]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725028" style="font-weight: bold;text-decoration: underline;">HA725028</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat CXCL1 protein (<a href="/products/HA211010" style="font-weight: bold;text-decoration: underline;">HA211010</a>) starting from 1000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725029" style="font-weight: bold;text-decoration: underline;">HA725029</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725029_2.png?v=20260401003030)
Sandwich ELISA analysis of Rat CXCL1 matched pair antibodies
Capture: HA725028, Mouse/Rat CXCL1/GRO Rabbit mAb [PSH13-14]
Detector: HA725029, Mouse/Rat CXCL1/GRO Rabbit mAb [PSH13-15]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725028) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat CXCL1 protein (HA211010) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA725029, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native CXCL1 in mouse samples.<br /><br />Capture: <a href="/products/HA725028" style="font-weight: bold;text-decoration: underline;">HA725028</a>, Mouse/Rat CXCL1/GRO Rabbit mAb [PSH13-14]<br />Detector: <a href="/products/HA725029" style="font-weight: bold;text-decoration: underline;">HA725029</a>, Mouse/Rat CXCL1/GRO Rabbit mAb [PSH13-15]<br /><br />Interpolated concentration of native CXCL1 was measured in duplicate at different sample concentrations and interpolated from the CXCL1 standard curves. Undiluted samples was100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 266 pg/mL in NIH/3T3.](https://storage.huabio.cn/huabio/productImg/HA725029_3.png?v=20260401003030)
Interpolated concentrations of native CXCL1 in mouse samples.
Capture: HA725028, Mouse/Rat CXCL1/GRO Rabbit mAb [PSH13-14]
Detector: HA725029, Mouse/Rat CXCL1/GRO Rabbit mAb [PSH13-15]
Interpolated concentration of native CXCL1 was measured in duplicate at different sample concentrations and interpolated from the CXCL1 standard curves. Undiluted samples was100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 266 pg/mL in NIH/3T3.
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