Biotin Conjugated Mouse/Rat IGF1 Recombinant Rabbit Monoclonal Antibody [PSH12-43] - Detector
Overview
Product Name
Biotin Conjugated Mouse/Rat IGF1 Recombinant Rabbit Monoclonal Antibody [PSH12-43] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse IGF1 aa 49-118 (HA210936).
Species Reactivity
Mouse, Rat
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Mouse IGF1 protein (HA210936). Recombinant Rat IGF1 protein (HA211009).
Conjugation
Biotin
Clone Number
PSH12-43
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-42] to Mouse/Rat IGF1 antibody (Capture) (HA725037) and Recombinant Mouse/Rat IGF1 protein (HA210936/HA211009) as the standard. The reference range value is 781-50,000 pg/mL.
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ELISA
-
Use at an assay dependent concentration.
Target
Function
Insulin-like growth factor 1 (IGF-1), also called somatomedin C, is a hormone similar in molecular structure to insulin which plays an important role in childhood growth, and has anabolic effects in adults. In the 1950s IGF-1 was called "sulfation factor" because it stimulated sulfation of cartilage in vitro, and in the 1970s due to its effects it was termed "nonsuppressible insulin-like activity" (NSILA). IGF-1 is a protein that in humans is encoded by the IGF1 gene. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 has a molecular weight of 7,649 daltons. In dogs, an ancient mutation in IGF1 is the primary cause of the toy phenotype. IGF-1 is produced primarily by the liver. Production is stimulated by growth hormone (GH). Most of IGF-1 is bound to one of 6 binding proteins (IGF-BP). IGFBP-1 is regulated by insulin. IGF-1 is produced throughout life; the highest rates of IGF-1 production occur during the pubertal growth spurt. The lowest levels occur in infancy and old age.
Background References
1. Werner H. The IGF1 Signaling Pathway: From Basic Concepts to Therapeutic Opportunities. Int J Mol Sci. 2023 Oct
2. Basu R et al. GH and IGF1 in cancer therapy resistance. Endocr Relat Cancer. 2023 Jul
Subcellular Location
Secreted.
Synonyms
IBP1 antibody
IGF I antibody
IGF IA antibody
IGF IB antibody
IGF-I antibody
Igf1 antibody
IGF1_HUMAN antibody
IGF1A antibody
IGFI antibody
IGFIA antibody
ExpandImages
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![Sandwich ELISA analysis of Mouse IGF1 matched pair antibodies<br /><br />Capture: <a href="/products/HA725037" style="font-weight: bold;text-decoration: underline;">HA725037</a>, Mouse/Rat IGF1 Rabbit mAb [PSH12-42]<br />Detector: <a href="/products/HA725038" style="font-weight: bold;text-decoration: underline;">HA725038</a>, Mouse/Rat IGF1 Rabbit mAb [PSH12-43]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725037" style="font-weight: bold;text-decoration: underline;">HA725037</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IGF1 protein (<a href="/products/HA210936" style="font-weight: bold;text-decoration: underline;">HA210936</a>) starting from 50000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725038" style="font-weight: bold;text-decoration: underline;">HA725038</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725039B_1.png?v=20260604174822)
Sandwich ELISA analysis of Mouse IGF1 matched pair antibodies
Capture: HA725037, Mouse/Rat IGF1 Rabbit mAb [PSH12-42]
Detector: HA725038, Mouse/Rat IGF1 Rabbit mAb [PSH12-43]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725037) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IGF1 protein (HA210936) starting from 50000 pg/ml to 0 pg/ml and detect antibody (HA725038, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Sandwich ELISA analysis of Rat IGF1 matched pair antibodies<br /><br />Capture: <a href="/products/HA725037" style="font-weight: bold;text-decoration: underline;">HA725037</a>, Mouse/Rat IGF1 Rabbit mAb [PSH12-42]<br />Detector: <a href="/products/HA725038" style="font-weight: bold;text-decoration: underline;">HA725038</a>, Mouse/Rat IGF1 Rabbit mAb [PSH12-43]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725037" style="font-weight: bold;text-decoration: underline;">HA725037</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat IGF1 protein (<a href="/products/HA211009" style="font-weight: bold;text-decoration: underline;">HA211009</a>) starting from 50000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725038" style="font-weight: bold;text-decoration: underline;">HA725038</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725039B_2.png?v=20260604174822)
Sandwich ELISA analysis of Rat IGF1 matched pair antibodies
Capture: HA725037, Mouse/Rat IGF1 Rabbit mAb [PSH12-42]
Detector: HA725038, Mouse/Rat IGF1 Rabbit mAb [PSH12-43]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725037) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat IGF1 protein (HA211009) starting from 50000 pg/ml to 0 pg/ml and detect antibody (HA725038, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native Mouse IGF1 in mouse samples.<br /><br />Capture: <a href="/products/HA725037" style="font-weight: bold;text-decoration: underline;">HA725037</a>, Mouse/Rat IGF1 Rabbit mAb [PSH12-42]<br />Detector: <a href="/products/HA725038" style="font-weight: bold;text-decoration: underline;">HA725038</a>, Mouse/Rat IGF1 Rabbit mAb [PSH12-43]<br /><br />Interpolated concentration of native IGF1 was measured in duplicate at different sample concentrations. Undiluted samples were 1.25% mouse serum. The mean IGF1 concentration was determined to be 1,584,637 pg/mL in mouse serum.](https://storage.huabio.cn/huabio/productImg/HA725039B_3.png?v=20260604174822)
Interpolated concentrations of native Mouse IGF1 in mouse samples.
Capture: HA725037, Mouse/Rat IGF1 Rabbit mAb [PSH12-42]
Detector: HA725038, Mouse/Rat IGF1 Rabbit mAb [PSH12-43]
Interpolated concentration of native IGF1 was measured in duplicate at different sample concentrations. Undiluted samples were 1.25% mouse serum. The mean IGF1 concentration was determined to be 1,584,637 pg/mL in mouse serum.
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