Biotin Conjugated Mouse IL-17E/IL-25 Recombinant Rabbit Monoclonal Antibody [PSH15-34] - Detector
Overview
Product Name
Biotin Conjugated Mouse IL-17E/IL-25 Recombinant Rabbit Monoclonal Antibody [PSH15-34] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse IL-17E/IL-25 aa 17-169 (HA211130).
Species Reactivity
Mouse
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Mouse IL-17E/IL-25 protein (HA211130).
Conjugation
Biotin
Clone Number
PSH15-34
Product Features
Form
Liquid
Concentration
1 mg/mL(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH15-26] to Mouse IL-17E/IL-25 antibody (Capture) (HA725043) and Recombinant Mouse IL-17E/IL-25 protein (HA211130) as the standard. The reference range value is 78.1-10,000 pg/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
Cytokine produced by various cells such as eosinophils, T-helper type 2 (Th2) cells or epithelial cells that plays a role in internal safety of adaptive immune responses by regulating cytokine production. Promotes and augments T-helper type 2 responses locally or systemically. Exerts its activity via its receptor composed of IL17RA and IL17RB for signal transduction. In turn, stimulates the JAK2-STAT5A pathway and promotes the secretion of type-2 associated cytokines including IL4, IL9 and IL13. Also induces the release of IL8, and IL6 from eosinophils through the combined activation of MAPK and NF-kappa-B pathways. Inhibits the differentiation of T-helper (Th17) cells via the production of IL4, IL5 and IL13.
Background References
1. Peng B., Sun L., Zhang M., Yan H., Shi G., Xia Z., Dai R., Tang W. Role of IL-25 on Eosinophils in the Initiation of Th2 Responses in Allergic Asthma. Front. Immunol. 13:842500-842500 (2022)
2. Rickel E.A., Siegel L.A., Yoon B.R., Rottman J.B., Kugler D.G., Swart D.A., Anders P.M., Tocker J.E., Comeau M.R., Budelsky A.L. Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities. J. Immunol. 181:4299-4310 (2008)
Subcellular Location
Secreted.
UNIPROT
Synonyms
IL 17E antibody
IL 25 antibody
IL-17E antibody
IL-25 antibody
IL17e antibody
IL25 antibody
IL25_HUMAN antibody
Interleukin 17E antibody
Interleukin 25 antibody
Interleukin-17E antibody
ExpandImages
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![Sandwich ELISA analysis of Mouse IL-17E/IL-25 matched pair antibodies<br /><br />Capture: <a href="/products/HA725043" style="font-weight: bold;text-decoration: underline;">HA725043</a>, Mouse IL-17E/IL-25 Rabbit mAb [PSH15-33]<br />Detector: <a href="/products/HA725044" style="font-weight: bold;text-decoration: underline;">HA725044</a>, Mouse IL-17E/IL-25 Rabbit mAb [PSH15-34]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725043" style="font-weight: bold;text-decoration: underline;">HA725043</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-17E/IL-25 protein (<a href="/products/HA211130" style="font-weight: bold;text-decoration: underline;">HA211130</a>) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725044" style="font-weight: bold;text-decoration: underline;">HA725044</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725045B_1.png?v=20260604174756)
Sandwich ELISA analysis of Mouse IL-17E/IL-25 matched pair antibodies
Capture: HA725043, Mouse IL-17E/IL-25 Rabbit mAb [PSH15-33]
Detector: HA725044, Mouse IL-17E/IL-25 Rabbit mAb [PSH15-34]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725043) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-17E/IL-25 protein (HA211130) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725044, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
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