Biotin Conjugated Human CCL5 Recombinant Rabbit Monoclonal Antibody [PSH13-17] - Detector
Overview
Product Name
Biotin Conjugated Human CCL5 Recombinant Rabbit Monoclonal Antibody [PSH13-17] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human CCL5 aa 24-91 (HA210998).
Species Reactivity
Human, Dog, Cynomolgus monkey
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human CCL5 protein (HA210998).
Conjugation
Biotin
Clone Number
PSH13-17
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-16] to Human CCL5 antibody (Capture) (HA725055) and recombinant Human CCL5 protein (HA210998) as the standard. The reference range value is 7.8-1,000 pg/mL.
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ELISA
-
Use at an assay dependent concentration.
Target
Function
Chemokine (C-C motif) ligand 5 (also CCL5) is a protein which in humans is encoded by the CCL5 gene. The gene has been discovered in 1990 by in situ hybridisation and it is localised on 17q11.2-q12 chromosome. It is also known as RANTES (regulated on activation, normal T-cell expressed and secreted). CCL5 belongs to the CC subfamily of chemokines, due to its adjacent cysteines near N terminus. It is an 8kDa protein acting as a classical chemotactic cytokine or chemokine. It consists of 68 amino acids. CCL5 is proinflammatory chemokine, recruiting leukocytes to the site of inflammation. It is chemotactic for T cells, eosinophils, and basophils, but also for monocytes, natural-killer (NK) cells, dendritic cells and mastocytes. With the help of particular cytokines (i.e., IL-2 and IFN-γ) that are released by T cells, CCL5 also induces the proliferation and activation of certain NK cells to form CHAK (CC-Chemokine-activated killer) cells. It is also an HIV-suppressive factor released from CD8+ T cells
Background References
1. Zeng Z et al. CCL5/CCR5 axis in human diseases and related treatments. Genes Dis. 2022 Jan
2. Chen K et al. Single cell RNA-seq reveals the CCL5/SDC1 receptor-ligand interaction between T cells and tumor cells in pancreatic cancer. Cancer Lett. 2022 Oct
Subcellular Location
Secreted.
UNIPROT
Synonyms
Beta chemokine RANTES antibody
Beta chemokine RANTES precursor antibody
C C motif chemokine 5 antibody
CCL 5 antibody
CCL5 antibody
CCL5_HUMAN antibody
Chemokine (C C motif) ligand 5 antibody
Chemokine CC Motif Ligand 5 antibody
D17S136E antibody
EoCP antibody
ExpandImages
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![Sandwich ELISA analysis of Human CCL5 matched pair antibodies<br /><br />Capture: <a href="/products/HA725055" style="font-weight: bold;text-decoration: underline;">HA725055</a>, Human CCL5 Rabbit mAb [PSH13-16]<br />Detector: <a href="/products/HA725056" style="font-weight: bold;text-decoration: underline;">HA725056</a>, Human CCL5 Rabbit mAb [PSH13-17]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725055" style="font-weight: bold;text-decoration: underline;">HA725055</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL5 protein (<a href="/products/HA210998" style="font-weight: bold;text-decoration: underline;">HA210998</a>) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725056" style="font-weight: bold;text-decoration: underline;">HA725056</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725057B_1.png?v=20260604174816)
Sandwich ELISA analysis of Human CCL5 matched pair antibodies
Capture: HA725055, Human CCL5 Rabbit mAb [PSH13-16]
Detector: HA725056, Human CCL5 Rabbit mAb [PSH13-17]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725055) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL5 protein (HA210998) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA725056, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native CCL5 in human samples.<br /><br />Capture: <a href="/products/HA725055" style="font-weight: bold;text-decoration: underline;">HA725055</a>, Human CCL5 Rabbit mAb [PSH13-16]<br />Detector: <a href="/products/HA725056" style="font-weight: bold;text-decoration: underline;">HA725056</a>, Human CCL5 Rabbit mAb [PSH13-17]<br /><br />Interpolated concentration of native CCL5 was measured in duplicate at different sample concentrations and interpolated from the CCL5 standard curves. Undiluted samples were 2% human serum and 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CCL5 concentration was determined to be 11,647 pg/mL in NK-92 and 182 pg/ml<br /> in Raji cell supernatant.](https://storage.huabio.cn/huabio/productImg/HA725057B_2.png?v=20260604174816)
Interpolated concentrations of native CCL5 in human samples.
Capture: HA725055, Human CCL5 Rabbit mAb [PSH13-16]
Detector: HA725056, Human CCL5 Rabbit mAb [PSH13-17]
Interpolated concentration of native CCL5 was measured in duplicate at different sample concentrations and interpolated from the CCL5 standard curves. Undiluted samples were 2% human serum and 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CCL5 concentration was determined to be 11,647 pg/mL in NK-92 and 182 pg/ml
in Raji cell supernatant.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"