Biotin Conjugated Human ANGPTL3 Recombinant Rabbit Monoclonal Antibody [PSH17-40] - Detector

Sandwich ELISA analysis of Human ANGPTL3 matched pair antibodies

Capture: HA725079, Human ANGPTL3 Rabbit mAb [PSH17-39]
Detector: HA725080, Human ANGPTL3 Rabbit mAb [PSH17-40]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725079) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ANGPTL3 protein (HA211029) protein starting from 25,000 pg/ml to 0 pg/ml and detect antibody (HA725080, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native ANGPTL3 in Human serum.

Capture: HA725079, Human ANGPTL3 Rabbit mAb [PSH17-39]
Detector: HA725080, Human ANGPTL3 Rabbit mAb [PSH17-40]

Interpolated concentration of native ANGPTL3 was measured in duplicate at different sample concentrations and interpolated from the ANGPTL3 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean ANGPTL3 concentration was determined to be 262,537 pg/ml in Human serum.