Human YKL-40 Recombinant Rabbit Monoclonal Antibody [PSH13-09] - BSA and Azide free (Detector)
Overview
Product Name
Human YKL-40 Recombinant Rabbit Monoclonal Antibody [PSH13-09] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human YKL-40 aa 22-383 (HA211030).
Species Reactivity
Human, Cynomolgus monkey
Validated Applications
ELISA(Det)
Positive Control
Recombinant Human YKL-40 protein (HA211030).
Conjugation
unconjugated
Clone Number
PSH13-09
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-08] to Human YKL-40 antibody (Capture) (HA725088) and Recombinant Human YKL-40 protein (HA211030) as the standard. The reference range value is 31.3-4,000 pg/ml.
Target
Function
Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.
Background References
1. Connolly K et al. Potential role of chitinase-3-like protein 1 (CHI3L1/YKL-40) in neurodegeneration and Alzheimer\'s disease. Alzheimers Dement. 2023 Jan
2. Yu JE et al. Significance of chitinase-3-like protein 1 in the pathogenesis of inflammatory diseases and cancer. Exp Mol Med. 2024 Feb
Subcellular Location
Secreted, extracellular space, Cytoplasm, perinuclear region, Endoplasmic reticulum.
UNIPROT
Synonyms
39 kDa synovial protein antibody
ASRT7 antibody
Cartilage glycoprotein 39 antibody
CGP-39 antibody
CGP39 antibody
CH3L1_HUMAN antibody
CHI3L1 antibody
chitinase 3 like 1 (cartilage glycoprotein 39) antibody
chitinase 3 like 1 antibody
Chitinase 3 like protein 1 precursor antibody
ExpandImages
-
![Sandwich ELISA analysis of Human YKL-40 matched pair antibodies<br /><br />Capture: <a href="/products/HA725088" style="font-weight: bold;text-decoration: underline;">HA725088</a>, Human YKL-40 Rabbit mAb [PSH13-08]<br />Detector: <a href="/products/HA725089" style="font-weight: bold;text-decoration: underline;">HA725089</a>, Human YKL-40 Rabbit mAb [PSH13-09]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725088" style="font-weight: bold;text-decoration: underline;">HA725088</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human YKL-40 protein (<a href="/products/HA211030" style="font-weight: bold;text-decoration: underline;">HA211030</a>) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725089" style="font-weight: bold;text-decoration: underline;">HA725089</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725089_1.png?v=20260429162226)
Sandwich ELISA analysis of Human YKL-40 matched pair antibodies
Capture: HA725088, Human YKL-40 Rabbit mAb [PSH13-08]
Detector: HA725089, Human YKL-40 Rabbit mAb [PSH13-09]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725088) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human YKL-40 protein (HA211030) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725089, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native YKL-40 in U87 MG extract samples based on a 1000 µg/ml extract load.<br /><br />Capture: <a href="/products/HA725088" style="font-weight: bold;text-decoration: underline;">HA725088</a>, Human YKL-40 Rabbit mAb [PSH13-08]<br />Detector: <a href="/products/HA725089" style="font-weight: bold;text-decoration: underline;">HA725089</a>, Human YKL-40 Rabbit mAb [PSH13-09]<br /><br />Interpolated concentration of native YKL-40 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean YKL-40 concentration was determined to be 15973 pg/mL in U-87 MG cell extract.](https://storage.huabio.cn/huabio/productImg/HA725089_2.png?v=20260429162226)
Interpolated concentrations of native YKL-40 in U87 MG extract samples based on a 1000 µg/ml extract load.
Capture: HA725088, Human YKL-40 Rabbit mAb [PSH13-08]
Detector: HA725089, Human YKL-40 Rabbit mAb [PSH13-09]
Interpolated concentration of native YKL-40 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean YKL-40 concentration was determined to be 15973 pg/mL in U-87 MG cell extract.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"