Mouse/Rat Complement C3 Recombinant Rabbit Monoclonal Antibody [PSH16-82] - BSA and Azide free (Detector)
Overview
Product Name
Mouse/Rat Complement C3 Recombinant Rabbit Monoclonal Antibody [PSH16-82] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse Complement C3 aa 25-1663 (HA211093).
Species Reactivity
Mouse, Rat
Validated Applications
ELISA(Det)
Positive Control
Recombinant Mouse Complement C3 protein (HA211093).
Conjugation
unconjugated
Clone Number
PSH16-82
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-81] to Mouse/Rat Complement C3 antibody (Capture) (HA725143) and Recombinant Mouse Complement C3 protein (HA211093) as the standard. The reference range value is 312.5-40,000 pg/mL.
Target
Function
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. Defects in C3 are the cause of complement component 3 deficiency (C3D). Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9). Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5).
Background References
1. Murayama MA et al. CTRP6 is an endogenous complement regulator that can effectively treat induced arthritis. Nat Commun 6:8483 (2015).
2. Lee MS et al. Exploitation of the complement system by oncogenic Kaposi\'s sarcoma-associated herpesvirus for cell survival and persistent infection. PLoS Pathog 10:e1004412 (2014).
Subcellular Location
Secreted.
Synonyms
Acylation stimulating protein cleavage product antibody
AHUS5 antibody
ARMD9 antibody
ASP antibody
C3 and PZP like alpha 2 macroglobulin domain containing protein 1 antibody
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
C3 antibody
CO3_HUMAN antibody
Complement C3 antibody
Complement C3c alpha' chain fragment 2 antibody
ExpandImages
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![Sandwich ELISA analysis of Mouse Complement C3 matched pair antibodies<br /><br />Capture: <a href="/products/HA725143" style="font-weight: bold;text-decoration: underline;">HA725143</a>, Mouse/Rat Complement C3 Rabbit mAb [PSH16-81]<br />Detector: <a href="/products/HA725144" style="font-weight: bold;text-decoration: underline;">HA725144</a>, Mouse/Rat Complement C3 Rabbit mAb [PSH16-82]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA725143" style="font-weight: bold;text-decoration: underline;">HA725143</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Complement C3 protein (<a href="/products/HA211093" style="font-weight: bold;text-decoration: underline;">HA211093</a>) protein starting from 40,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725144" style="font-weight: bold;text-decoration: underline;">HA725144</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725144_1.png?v=20260527093248)
Sandwich ELISA analysis of Mouse Complement C3 matched pair antibodies
Capture: HA725143, Mouse/Rat Complement C3 Rabbit mAb [PSH16-81]
Detector: HA725144, Mouse/Rat Complement C3 Rabbit mAb [PSH16-82]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725143) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Complement C3 protein (HA211093) protein starting from 40,000 pg/ml to 0 pg/ml and detect antibody (HA725144, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native Complement C3 in mouse serum samples.<br /><br />Capture: <a href="/products/HA725143" style="font-weight: bold;text-decoration: underline;">HA725143</a>, Mouse/Rat Complement C3 Rabbit mAb [PSH16-81]<br />Detector: <a href="/products/HA725144" style="font-weight: bold;text-decoration: underline;">HA725144</a>, Mouse/Rat Complement C3 Rabbit mAb [PSH16-82]<br /><br />The concentrations of Complement C3 were measured in triplicates, interpolated from the Complement C3 standard curve and corrected for sample dilution. Undiluted samples are mouse serum 0.0005%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean Complement C3 concentration was determined to be 1,269 µg/ml in mouse serum.](https://storage.huabio.cn/huabio/productImg/HA725144_2.png?v=20260527093248)
Interpolated concentrations of native Complement C3 in mouse serum samples.
Capture: HA725143, Mouse/Rat Complement C3 Rabbit mAb [PSH16-81]
Detector: HA725144, Mouse/Rat Complement C3 Rabbit mAb [PSH16-82]
The concentrations of Complement C3 were measured in triplicates, interpolated from the Complement C3 standard curve and corrected for sample dilution. Undiluted samples are mouse serum 0.0005%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean Complement C3 concentration was determined to be 1,269 µg/ml in mouse serum. -
![Interpolated concentrations of native Complement C3 in rat serum samples.<br /><br />Capture: <a href="/products/HA725143" style="font-weight: bold;text-decoration: underline;">HA725143</a>, Mouse/Rat Complement C3 Rabbit mAb [PSH16-81]<br />Detector: <a href="/products/HA725144" style="font-weight: bold;text-decoration: underline;">HA725144</a>, Mouse/Rat Complement C3 Rabbit mAb [PSH16-82]<br /><br />The concentrations of Complement C3 were measured in duplicates, interpolated from the Complement C3 standard curve and corrected for sample dilution. Undiluted samples are rat serum 0.000313%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C3 concentration was determined to be 3,772 µg/ml in rat serum.](https://storage.huabio.cn/huabio/productImg/HA725144_3.png?v=20260527093248)
Interpolated concentrations of native Complement C3 in rat serum samples.
Capture: HA725143, Mouse/Rat Complement C3 Rabbit mAb [PSH16-81]
Detector: HA725144, Mouse/Rat Complement C3 Rabbit mAb [PSH16-82]
The concentrations of Complement C3 were measured in duplicates, interpolated from the Complement C3 standard curve and corrected for sample dilution. Undiluted samples are rat serum 0.000313%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C3 concentration was determined to be 3,772 µg/ml in rat serum.
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