H-FABP Recombinant Rabbit Monoclonal Antibody [PSH16-98] - BSA and Azide free (Capture)
Overview
Product Name
H-FABP Recombinant Rabbit Monoclonal Antibody [PSH16-98] - BSA and Azide free (Capture)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human H-FABP aa 1-133 (HA211159).
Species Reactivity
Human, Mouse, Cynomolgus monkey
Validated Applications
ELISA(Cap)
Positive Control
Recombinant Human H-FABP protein (HA211159).
Conjugation
unconjugated
Clone Number
PSH16-98
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-99] to Human H-FABP antibody (Detector) (HA725178) and Recombinant Human H-FABP protein (HA211159) as the standard. The reference range value is 15.6-2,000 pg/mL.
Target
Function
Heart-type fatty acid binding protein (hFABP) also known as mammary-derived growth inhibitor is a protein that in humans is encoded by the FABP3 gene. Heart-type Fatty Acid-Binding Protein (H-FABP) is a small cytoplasmic protein (15 kDa) released from cardiac myocytes following an ischemic episode. Like the nine other distinct FABPs that have been identified, H-FABP is involved in active fatty acid metabolism where it transports fatty acids from the cell membrane to mitochondria for oxidation. The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is also a candidate tumor suppressor gene for human breast cancer.
Background References
1. Wang L et al. Structural Insights into Mouse H-FABP. Life (Basel). 2022 Sep
2. Guamán-Pilco D et al. H-FABP as a Biomarker in Transient Ischemic Attack. J Cardiovasc Transl Res. 2025 Feb
Subcellular Location
Cytoplasm.
Synonyms
422 protein antibody
Cardiac FABP antibody
Cardiac Fatty Acid Binding Protein antibody
FABP 11 antibody
FABP 3 antibody
FABP11 antibody
FABP3 antibody
FABPH_HUMAN antibody
Fatty acid binding protein 11 antibody
Fatty acid binding protein 3 antibody
ExpandImages
-
![Sandwich ELISA analysis of Human H-FABP matched pair antibodies<br /><br />Capture: <a href="/products/HA725177" style="font-weight: bold;text-decoration: underline;">HA725177</a>, Human H-FABP Rabbit mAb [PSH16-98]<br />Detector: <a href="/products/HA725178" style="font-weight: bold;text-decoration: underline;">HA725178</a>, Human H-FABP Rabbit mAb [PSH16-99]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA725177" style="font-weight: bold;text-decoration: underline;">HA725177</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human H-FABP protein (<a href="/products/HA211159" style="font-weight: bold;text-decoration: underline;">HA211159</a>) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725178" style="font-weight: bold;text-decoration: underline;">HA725178</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725177_1.png?v=20260401003131)
Sandwich ELISA analysis of Human H-FABP matched pair antibodies
Capture: HA725177, Human H-FABP Rabbit mAb [PSH16-98]
Detector: HA725178, Human H-FABP Rabbit mAb [PSH16-99]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725177) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human H-FABP protein (HA211159) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725178, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native H-FABP in human serum and THP-1 cell supernatant.<br /><br />Capture: <a href="/products/HA725177" style="font-weight: bold;text-decoration: underline;">HA725177</a>, Human H-FABP Rabbit mAb [PSH16-98]<br />Detector: <a href="/products/HA725178" style="font-weight: bold;text-decoration: underline;">HA725178</a>, Human H-FABP Rabbit mAb [PSH16-99]<br /><br />The concentrations of H-FABP were measured in duplicates, interpolated from the H-FABP standard curve and corrected for sample dilution. Undiluted samples are human serum 50% and THP-1 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 2,876 pg/ml in human serum and undetectable in THP-1 cell culture supernatant.](https://storage.huabio.cn/huabio/productImg/HA725177_2.png?v=20260401003131)
Interpolated concentrations of native H-FABP in human serum and THP-1 cell supernatant.
Capture: HA725177, Human H-FABP Rabbit mAb [PSH16-98]
Detector: HA725178, Human H-FABP Rabbit mAb [PSH16-99]
The concentrations of H-FABP were measured in duplicates, interpolated from the H-FABP standard curve and corrected for sample dilution. Undiluted samples are human serum 50% and THP-1 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 2,876 pg/ml in human serum and undetectable in THP-1 cell culture supernatant. -
![Interpolated concentrations of native H-FABP in mouse heart extract samples based on a 1,000 μg/ml extract load.<br /><br />Capture: <a href="/products/HA725177" style="font-weight: bold;text-decoration: underline;">HA725177</a>, Human H-FABP Rabbit mAb [PSH16-98]<br />Detector: <a href="/products/HA725178" style="font-weight: bold;text-decoration: underline;">HA725178</a>, Human H-FABP Rabbit mAb [PSH16-99]<br /><br />The concentrations of H-FABP were measured in duplicates, interpolated from the H-FABP standard curve and corrected for sample dilution. Undiluted samples are mouse heart extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 316 pg/ml in mouse heart extract.](https://storage.huabio.cn/huabio/productImg/HA725177_3.png?v=20260401003131)
Interpolated concentrations of native H-FABP in mouse heart extract samples based on a 1,000 μg/ml extract load.
Capture: HA725177, Human H-FABP Rabbit mAb [PSH16-98]
Detector: HA725178, Human H-FABP Rabbit mAb [PSH16-99]
The concentrations of H-FABP were measured in duplicates, interpolated from the H-FABP standard curve and corrected for sample dilution. Undiluted samples are mouse heart extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 316 pg/ml in mouse heart extract.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"