Biotin Conjugated Human Neurogranin Recombinant Rabbit Monoclonal Antibody [PSH16-57] - Detector
Overview
Product Name
Biotin Conjugated Human Neurogranin Recombinant Rabbit Monoclonal Antibody [PSH16-57] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Neurogranin aa 1-78 (HA211156).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human Neurogranin protein (HA211156).
Conjugation
Biotin
Clone Number
PSH16-57
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-56] to Human Neurogranin antibody (Capture) (HA725191) and Recombinant Human Neurogranin protein (HA210865) as the standard. The reference range value is 7.8-4,000 pg/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
Neurogranin is a calmodulin-binding protein expressed primarily in the brain, particularly in dendritic spines, and participating in the protein kinase C signaling pathway. Neurogranin has recently been found in aortic endothelial cells and cardiomyocytes. Neurogranin is the main postsynaptic protein regulating the availability of calmodulin, binding to it in the absence of calcium. Phosphorylation by protein kinase C lowers its binding ability. NRGN gene expression is controlled by thyroid hormones. Human neurogranin consists of 78 amino acids. One study tells of potential link of neurogranin gene to the heightened risk of schizophrenia in males, another study gives evidence of lowered neurogranin immunoreactivity in the brains of people suffering from schizophrenia. Neurogranin concentration in cerebrospinal fluid (CSF) is further discussed as marker for synaptic dysfunction in age-related neurodegeneration. It has also been shown to be specifically increased in patients with Alzheimer's disease. Especially, the ratio of CSF neurogranin trunc P75 and the beta-secretase BACE1 is suggested as a potential marker for cognitive deterioration in the progress of Alzheimer's disease.
Background References
1. Xiang Y et al. Neurogranin: A Potential Biomarker of Neurological and Mental Diseases. Front Aging Neurosci. 2020 Oct
2. Jorgensen AN et al. Neurogranin regulates calcium-dependent cardiac hypertrophy. Exp Mol Pathol. 2022 Aug
Subcellular Location
Axon, cytosol, dendritic spine head, glutamatergic synapse, mitochondrial membrane, neuronal cell body, postsynaptic membrane, trans-Golgi network transport vesicle membrane.
UNIPROT
Synonyms
Protein kinase C substrate RC3 antibody
Calmodulin binding protein antibody
Hng antibody
NEUG(55-78) antibody
NEUG_HUMAN antibody
Neurogranin (protein kinase C substrate) antibody
Ng antibody
NRGN antibody
Protein kinase C substrate antibody
RC3 antibody
Images
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![Sandwich ELISA analysis of Human Neurogranin matched pair antibodies<br /><br />Capture: <a href="/products/HA725191" style="font-weight: bold;text-decoration: underline;">HA725191</a>, Human Neurogranin Rabbit mAb [PSH16-56]<br />Detector: <a href="/products/HA725192" style="font-weight: bold;text-decoration: underline;">HA725192</a>, Human Neurogranin Rabbit mAb [PSH16-57]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA725191" style="font-weight: bold;text-decoration: underline;">HA725191</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Neurogranin protein (<a href="/products/HA211156" style="font-weight: bold;text-decoration: underline;">HA211156</a>) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725192" style="font-weight: bold;text-decoration: underline;">HA725192</a>, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725192B_1.png?v=20260604174736)
Sandwich ELISA analysis of Human Neurogranin matched pair antibodies
Capture: HA725191, Human Neurogranin Rabbit mAb [PSH16-56]
Detector: HA725192, Human Neurogranin Rabbit mAb [PSH16-57]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725191) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Neurogranin protein (HA211156) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725192, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"