Human CCL2 / MCP-1 Recombinant Rabbit Monoclonal Antibody [PSH18-22] - BSA and Azide free (Detector)
Overview
Product Name
Human CCL2 / MCP-1 Recombinant Rabbit Monoclonal Antibody [PSH18-22] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human CCL2 / MCP-1 aa 24-99 (HA210787).
Species Reactivity
Human, Cynomolgus monkey
Validated Applications
ELISA(Det)
Positive Control
Recombinant Human CCL2 / MCP-1 protein (HA210787).
Conjugation
unconjugated
Clone Number
PSH18-22
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH18-21] to Human CCL2 / MCP-1 antibody (Capture) (HA725195) and Recombinant Human CCL2 / MCP-1 protein (HA210787) as the standard. The reference range value is 7.8-4,000 pg/mL.
Target
Function
The chemokine (C-C motif) ligand 2 (CCL2) is also referred to as monocyte chemoattractant protein 1 (MCP1) and small inducible cytokine A2. CCL2 is a small cytokine that belongs to the CC chemokine family. CCL2 tightly regulates cellular mechanics and thereby recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection. CCL2 is a monomeric polypeptide, with a molecular weight of approximately 13-15 kDa depending on levels of glycosylation. CCL2 is anchored in the plasma membrane of endothelial cells by glycosaminoglycan side chains of proteoglycans. CCL2 is primarily secreted by monocytes, macrophages and dendritic cells. Platelet derived growth factor is a major inducer of CCL2 gene.
Background References
1. Xu M et al. Role of the CCL2-CCR2 signalling axis in cancer: Mechanisms and therapeutic targeting. Cell Prolif. 2021 Oct
2. Zhang H et al. Role of the CCL2-CCR2 axis in cardiovascular disease: Pathogenesis and clinical implications. Front Immunol. 2022 Aug
Subcellular Location
Secreted.
UNIPROT
Synonyms
C-C motif chemokine 2 antibody
CCL2 antibody
CCL2_HUMAN antibody
Chemokine (C C motif) ligand 2 antibody
GDCF 2 antibody
GDCF-2 antibody
GDCF2 antibody
HC11 antibody
HSMCR30 antibody
JE antibody
ExpandImages
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![Sandwich ELISA analysis of human CCL2(MCP1) matched pair antibodies<br /><br />Capture: <a href="/products/HA725195" style="font-weight: bold;text-decoration: underline;">HA725195</a>, Human CCL2 / MCP-1 Rabbit mAb [PSH18-21]<br />Detector: <a href="/products/HA725196" style="font-weight: bold;text-decoration: underline;">HA725196</a>, Human CCL2 / MCP-1 Rabbit mAb [PSH18-22]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA725195" style="font-weight: bold;text-decoration: underline;">HA725195</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL2 / MCP-1 protein (<a href="/products/HA210787" style="font-weight: bold;text-decoration: underline;">HA210787</a>) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725196" style="font-weight: bold;text-decoration: underline;">HA725196</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725196_1.png?v=20260401003138)
Sandwich ELISA analysis of human CCL2(MCP1) matched pair antibodies
Capture: HA725195, Human CCL2 / MCP-1 Rabbit mAb [PSH18-21]
Detector: HA725196, Human CCL2 / MCP-1 Rabbit mAb [PSH18-22]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725195) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL2 / MCP-1 protein (HA210787) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725196, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native CCL2(MCP1) in stimulated and unstimulated THP-1 extract samples based on a 1,000 µg/ml extract load.<br /><br />Capture: <a href="/products/HA725195" style="font-weight: bold;text-decoration: underline;">HA725195</a>, Human CCL2 / MCP-1 Rabbit mAb [PSH18-21]<br />Detector: <a href="/products/HA725196" style="font-weight: bold;text-decoration: underline;">HA725196</a>, Human CCL2 / MCP-1 Rabbit mAb [PSH18-22]<br /><br />THP-1 cells were treated with 80nM PMA for 24h then treated with 100ng/ml LPS for 4h then together with 1μg/ml BFA for another 3h. The concentrations of CCL2(MCP1) were measured in duplicates, interpolated from the CCL2(MCP1) standard curve and corrected for sample dilution. Undiluted samples are stimulated and unstimulated 33%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL2(MCP1) concentration was determined to be 1,287 pg/ml in stimulated and 207 pg/ml in unstimulated.](https://storage.huabio.cn/huabio/productImg/HA725196_2.png?v=20260401003138)
Interpolated concentrations of native CCL2(MCP1) in stimulated and unstimulated THP-1 extract samples based on a 1,000 µg/ml extract load.
Capture: HA725195, Human CCL2 / MCP-1 Rabbit mAb [PSH18-21]
Detector: HA725196, Human CCL2 / MCP-1 Rabbit mAb [PSH18-22]
THP-1 cells were treated with 80nM PMA for 24h then treated with 100ng/ml LPS for 4h then together with 1μg/ml BFA for another 3h. The concentrations of CCL2(MCP1) were measured in duplicates, interpolated from the CCL2(MCP1) standard curve and corrected for sample dilution. Undiluted samples are stimulated and unstimulated 33%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL2(MCP1) concentration was determined to be 1,287 pg/ml in stimulated and 207 pg/ml in unstimulated.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"