HRP Conjugated Human S100A12 Recombinant Rabbit Monoclonal Antibody [PSH17-68] - Detector
Overview
Product Name
HRP Conjugated Human S100A12 Recombinant Rabbit Monoclonal Antibody [PSH17-68] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human S100A12 aa 1-92 (HA211137).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human S100A12 protein (HA211137).
Conjugation
HRP
Clone Number
PSH17-68
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-67] to Human S100A12 antibody (Capture) (HA725215) and Recombinant Human S100A12 protein (HA211137) as the standard. The reference range value is 156-20,000 pg/mL.
-
ELISA
-
Use at an assay dependent concentration.
Target
Function
S100 calcium-binding protein A12 (S100A12) is a protein that in humans is encoded by the S100A12 gene. Human S100A12, also known as calgranulin C, was first described in 1995. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. Generally, S100A12 has a significant anti-infectious and antibacterial role that is related to its ability to uptake ions. For example, it inhibits the spread and virulence of H. pylori. Neutrophils and monocytes / macrophages are important source of S100A12 in the cell although some epithelial cells and dendritic cells are capable of its secretion. Some tissues are rich in these cells, and so in this protein. These include the spleen or lungs. It occurs intracellularly but is also produced into the extracellular environment where it occurs as a homodimer or hexamer.
Background References
1. Li Y et al. S100A12 as Biomarker of Disease Severity and Prognosis in Patients With Idiopathic Pulmonary Fibrosis. Front Immunol. 2022 Feb
2. Zhang X et al. S100A12 triggers NETosis to aggravate myocardial infarction injury via the Annexin A5-calcium axis. Nat Commun. 2025 Feb
Subcellular Location
Secreted, Cytoplasm, cytoskeleton, Cell membrane.
UNIPROT
Synonyms
CAAF1 antibody
CAGC antibody
Calcitermin antibody
Calcium-binding protein in amniotic fluid 1 antibody
Calgranulin C antibody
Calgranulin-C antibody
Calgranulin-related protein antibody
CGRP antibody
EN RAGE antibody
EN-RAGE antibody
ExpandImages
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![Sandwich ELISA analysis of Human S100A12 matched pair antibodies<br /><br />Capture: <a href="/products/HA725215" style="font-weight: bold;text-decoration: underline;">HA725215</a>, Human S100A12 Rabbit mAb [PSH17-67]<br />Detector: <a href="/products/HA725216" style="font-weight: bold;text-decoration: underline;">HA725216</a>, Human S100A12 Rabbit mAb [PSH17-68]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725215" style="font-weight: bold;text-decoration: underline;">HA725215</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human S100A12 protein (<a href="/products/HA211137" style="font-weight: bold;text-decoration: underline;">HA211137</a>) protein starting from 100,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725216" style="font-weight: bold;text-decoration: underline;">HA725216</a>, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725216H_1.png?v=20260604174726)
Sandwich ELISA analysis of Human S100A12 matched pair antibodies
Capture: HA725215, Human S100A12 Rabbit mAb [PSH17-67]
Detector: HA725216, Human S100A12 Rabbit mAb [PSH17-68]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725215) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human S100A12 protein (HA211137) protein starting from 100,000 pg/ml to 0 pg/ml and detect antibody (HA725216, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native S100A12 in Human serum.<br /><br />Capture: <a href="/products/HA725215" style="font-weight: bold;text-decoration: underline;">HA725215</a>, Human S100A12 Rabbit mAb [PSH17-67]<br />Detector: <a href="/products/HA725216" style="font-weight: bold;text-decoration: underline;">HA725216</a>, Human S100A12 Rabbit mAb [PSH17-68]<br /><br />Interpolated concentration of native S100A12 was measured in duplicate at different sample concentrations and interpolated from the S100A12 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean S100A12 concentration was determined to be 257,263 pg/ml in Human serum.](https://storage.huabio.cn/huabio/productImg/HA725216H_2.png?v=20260604174726)
Interpolated concentrations of native S100A12 in Human serum.
Capture: HA725215, Human S100A12 Rabbit mAb [PSH17-67]
Detector: HA725216, Human S100A12 Rabbit mAb [PSH17-68]
Interpolated concentration of native S100A12 was measured in duplicate at different sample concentrations and interpolated from the S100A12 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean S100A12 concentration was determined to be 257,263 pg/ml in Human serum.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Human S100A12 Recombinant Rabbit Monoclonal Antibody [PSH17-67] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human
Conjugate: unconjugated
Human S100A12 Recombinant Rabbit Monoclonal Antibody [PSH17-68] - BSA and Azide free (Detector)
Application: ELISA(Det)
Reactivity: Human
Conjugate: unconjugated
