SNAP25 Recombinant Rabbit Monoclonal Antibody [PSH17-66] - BSA and Azide free (Detector)
Overview
Product Name
SNAP25 Recombinant Rabbit Monoclonal Antibody [PSH17-66] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human SNAP25 aa 1-206 (HA211155).
Species Reactivity
Human, Mouse
Validated Applications
ELISA(Det)
Positive Control
Recombinant Human SNAP25 protein (HA211155).
Conjugation
unconjugated
Clone Number
PSH17-66
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-65] to SNAP25 antibody (Capture) (HA725227) and Recombinant Human SNAP25 protein (HA211155) as the standard. The reference range value is 15.6-4,000 pg/mL.
Target
Function
Synaptosomal-Associated Protein, 25kDa (SNAP-25) is a Target Soluble NSF (N-ethylmaleimide-sensitive factor) Attachment Protein Receptor (t-SNARE) protein. SNAP-25 is a component of the trans-SNARE complex, which accounts for membrane fusion specificity and directly executes fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. Molecular machinery driving exocytosis in neuromediator release. The core SNARE complex is formed by four α-helices contributed by synaptobrevin, syntaxin and SNAP-25. Synaptotagmin serves as a Ca2+ sensor and intimately regulates SNARE zipping. SNAP-25, a Q-SNARE protein, is anchored to the cytosolic face of membranes via palmitoyl side chains covalently bound to cysteine amino acid residues in the central linker domain of the molecule. This means that SNAP-25 does not contain a trans-membrane domain.
Background References
1. Wang W et al. Neuronal-specific TNFAIP1 ablation attenuates postoperative cognitive dysfunction via targeting SNAP25 for K48-linked ubiquitination. Cell Commun Signal. 2023 Dec
2. Chen Z et al. SNAP25-induced MYC upregulation promotes high-grade neuroendocrine lung carcinoma progression. Front Immunol. 2024 Oct
Subcellular Location
Cytoplasm, perinuclear region, Cell membrane; Lipid-anchor, Synapse, synaptosome, Photoreceptor inner segment.
Synonyms
bA416N4.2 antibody
Bdr antibody
CMS18 antibody
dJ1068F16.2 antibody
FLJ23079 antibody
HGNC:11132 antibody
MGC105414 antibody
MGC139754 antibody
Resistance to inhibitors of cholinesterase 4 homolog antibody
RIC 4 antibody
ExpandImages
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![Sandwich ELISA analysis of Human SNAP25 matched pair antibodies<br /><br />Capture: <a href="/products/HA725227" style="font-weight: bold;text-decoration: underline;">HA725227</a>, SNAP25 Rabbit mAb [PSH17-65]<br />Detector: <a href="/products/HA725228" style="font-weight: bold;text-decoration: underline;">HA725228</a>, SNAP25 Rabbit mAb [PSH17-66]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA725227" style="font-weight: bold;text-decoration: underline;">HA725227</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human SNAP25 protein (<a href="/products/HA211155" style="font-weight: bold;text-decoration: underline;">HA211155</a>) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725228" style="font-weight: bold;text-decoration: underline;">HA725228</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725228_1.png?v=20260429162303)
Sandwich ELISA analysis of Human SNAP25 matched pair antibodies
Capture: HA725227, SNAP25 Rabbit mAb [PSH17-65]
Detector: HA725228, SNAP25 Rabbit mAb [PSH17-66]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725227) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human SNAP25 protein (HA211155) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725228, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native SNAP25 in SH-SY5Y and PANC-1 extract samples based on a 1,000 μg/ml extract load.<br /><br />Capture: <a href="/products/HA725227" style="font-weight: bold;text-decoration: underline;">HA725227</a>, SNAP25 Rabbit mAb [PSH17-65]<br />Detector: <a href="/products/HA725228" style="font-weight: bold;text-decoration: underline;">HA725228</a>, SNAP25 Rabbit mAb [PSH17-66]<br /><br />The concentrations of SNAP25 were measured in duplicates, interpolated from the SNAP25 standard curve and corrected for sample dilution. Undiluted samples are SH-SY5Y extract 13% and PANC-1 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SNAL25 concentration was determined to be 2,561 pg/ml in SH-SY5Y extract and undetectable in PANC-1 extract.](https://storage.huabio.cn/huabio/productImg/HA725228_2.png?v=20260429162303)
Interpolated concentrations of native SNAP25 in SH-SY5Y and PANC-1 extract samples based on a 1,000 μg/ml extract load.
Capture: HA725227, SNAP25 Rabbit mAb [PSH17-65]
Detector: HA725228, SNAP25 Rabbit mAb [PSH17-66]
The concentrations of SNAP25 were measured in duplicates, interpolated from the SNAP25 standard curve and corrected for sample dilution. Undiluted samples are SH-SY5Y extract 13% and PANC-1 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SNAL25 concentration was determined to be 2,561 pg/ml in SH-SY5Y extract and undetectable in PANC-1 extract.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"