Human Angiotensinogen Recombinant Rabbit Monoclonal Antibody [PSH22-02] - BSA and Azide free (Capture)
Overview
Product Name
Human Angiotensinogen Recombinant Rabbit Monoclonal Antibody [PSH22-02] - BSA and Azide free (Capture)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Angiotensinogen aa 34-485 (HA211165).
Species Reactivity
Human
Validated Applications
ELISA(Cap)
Positive Control
Recombinant Human Angiotensinogen protein (HA211165).
Conjugation
unconjugated
Clone Number
PSH22-02
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH22-03] to Human Angiotensinogen antibody (Detector) (HA725246) and Recombinant Human Angiotensinogen protein (HA211165) as the standard. The reference range value is 78.13-40,000 pg/mL.
Target
Function
Angiotensin is formed from a precursor, angiotensinogen, which is produced by the liver and found in the a-globulin fraction of plasma. The lowering of blood pressure is a stimulus to secretion of Renin by the kidney into the blood. Renin cleaves from angiotensinogen a terminal decapeptide, Angiotensin I (Ang I). This is further altered by the enzymatic removal of a dipeptide to form Angiotensin II (Ang II). Screening a panel of human-mouse somatic cell hybrids confirmed the assignment of the AGT locus to human chromosome 1. Angiotensin, an octapeptide hormone, is an important physiological effector of blood pressure and volume regulation through vasoconstriction, aldosterone release, sodium uptake and thirst stimulation. It has been shown that mechanical stress causes release of Angiotensin from cardiac myocytes and that Angiotensin acts as an initial mediator of the hypertrophic response. Angiotensin treatment also stimulates phosphorylation of Shc, FAK and MAP kinases and induces MKP-1, indicating stimulation of growth factor pathways. Angiotensin stimulation through AT1 has been shown to activate the JAK/Stat pathway involving a direct interaction between JAK2 and AT1 as demonstrated by co-immunoprecipitation.
Background References
1. Daugherty A et al. Angiotensinogen as a Therapeutic Target for Cardiovascular and Metabolic Diseases. Arterioscler Thromb Vasc Biol. 2024 May
2. Cruz-López EO et al. Angiotensinogen Suppression: A New Tool to Treat Cardiovascular and Renal Disease. Hypertension. 2022 Oct
Subcellular Location
Secreted.
UNIPROT
Synonyms
Aangiotensinogen (serpin peptidase inhibitor clade A member 8) antibody
AGT antibody
AI265500 antibody
Alpha 1 antiproteinase antitrypsin antibody
Ang antibody
Ang I antibody
Ang II antibody
Ang III antibody
AngII antibody
Angiotensin I antibody
ExpandImages
-
![Sandwich ELISA analysis of Human Angiotensinogen matched pair antibodies<br /><br />Capture: <a href="/products/HA725245" style="font-weight: bold;text-decoration: underline;">HA725245</a>, Human Angiotensinogen Rabbit mAb [PSH22-02]<br />Detector: <a href="/products/HA725246" style="font-weight: bold;text-decoration: underline;">HA725246</a>, Human Angiotensinogen Rabbit mAb [PSH22-03]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA725245" style="font-weight: bold;text-decoration: underline;">HA725245</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Angiotensinogen protein (<a href="/products/HA211165" style="font-weight: bold;text-decoration: underline;">HA211165</a>) starting from 40,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725246" style="font-weight: bold;text-decoration: underline;">HA725246</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725245_1.png?v=20260401003158)
Sandwich ELISA analysis of Human Angiotensinogen matched pair antibodies
Capture: HA725245, Human Angiotensinogen Rabbit mAb [PSH22-02]
Detector: HA725246, Human Angiotensinogen Rabbit mAb [PSH22-03]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725245) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Angiotensinogen protein (HA211165) starting from 40,000 pg/ml to 0 pg/ml and detect antibody (HA725246, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native Angiotensinogen in human serum samples.<br /><br />Capture: <a href="/products/HA725245" style="font-weight: bold;text-decoration: underline;">HA725245</a>, Human Angiotensinogen Rabbit mAb [PSH22-02]<br />Detector: <a href="/products/HA725246" style="font-weight: bold;text-decoration: underline;">HA725246</a>, Human Angiotensinogen Rabbit mAb [PSH22-03]<br /><br />The concentrations of Angiotensinogen were measured in duplicates, interpolated from the Angiotensinogen standard curve and corrected for sample dilution. Undiluted samples are human serum 0.025%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiotensinogen concentration was determined to be 79.31 µg/ml in human serum.](https://storage.huabio.cn/huabio/productImg/HA725245_2.png?v=20260401003158)
Interpolated concentrations of native Angiotensinogen in human serum samples.
Capture: HA725245, Human Angiotensinogen Rabbit mAb [PSH22-02]
Detector: HA725246, Human Angiotensinogen Rabbit mAb [PSH22-03]
The concentrations of Angiotensinogen were measured in duplicates, interpolated from the Angiotensinogen standard curve and corrected for sample dilution. Undiluted samples are human serum 0.025%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiotensinogen concentration was determined to be 79.31 µg/ml in human serum.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Angiotensinogen Recombinant Rabbit Monoclonal Antibody [PSH21-83]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Human Angiotensinogen Recombinant Rabbit Monoclonal Antibody [PSH22-03] - BSA and Azide free (Detector)
Application: ELISA(Det)
Reactivity: Human
Conjugate: unconjugated
Angiotensinogen Recombinant Rabbit Monoclonal Antibody [SD201-02]
Application: WB,IF-Cell,IF-Tissue,IP
Reactivity: Human
Conjugate: unconjugated
Biotin Conjugated Human Angiotensinogen Recombinant Rabbit Monoclonal Antibody [PSH22-03] - Detector
Application: ELISA(Det),ELISA
Reactivity: Human
Conjugate: Biotin
