Biotin Conjugated Human CXCL2/MIP-2 Recombinant Rabbit Monoclonal Antibody [PSH17-89] - Detector
Overview
Product Name
Biotin Conjugated Human CXCL2/MIP-2 Recombinant Rabbit Monoclonal Antibody [PSH17-89] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human CXCL2/MIP-2 aa 35-107 (HA210904).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human CXCL2/MIP-2 protein (HA210904).
Conjugation
Biotin
Clone Number
PSH17-89
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-88] to Human CXCL2/MIP-2 antibody (Capture) (HA725247) and Recombinant Human CXCL2/MIP-2 protein (HA210904) as the standard. The reference range value is 15.6-1,000 pg/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
Chemokine (C-X-C motif) ligand 2 (CXCL2) is a small cytokine belonging to the CXC chemokine family that is also called macrophage inflammatory protein 2-alpha (MIP2-alpha), Growth-regulated protein beta (Gro-beta) and Gro oncogene-2 (Gro-2). CXCL2 is 90% identical in amino acid sequence as a related chemokine, CXCL1. This chemokine is secreted by monocytes and macrophages and is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. The gene for CXCL2 is located on human chromosome 4 in a cluster of other CXC chemokines. CXCL2 mobilizes cells by interacting with a cell surface chemokine receptor called CXCR2. CXCL2, like related chemokines, is also a powerful neutrophil chemoattractant and is involved in many immune responses including wound healing, cancer metastasis, and angiogenesis. A study was published in 2013 testing the role of CXCL2, CXCL3, and CXCL1 in the migration of airway smooth muscle cells (ASMCs) migration which plays a significant role in asthma. The results of this study showed that CXCL2 and CXCL3 both help with the mediation of normal and asthmatic ASMC migration through different mechanisms.
Background References
1. Liu Y et al. Single-cell immune profiling of mouse liver aging reveals Cxcl2+ macrophages recruit neutrophils to aggravate liver injury. Hepatology. 2024 Mar
2. Lin W et al. Early infiltrating NKT lymphocytes attenuate bone regeneration through secretion of CXCL2. Sci Adv. 2024 May
Subcellular Location
Secreted.
UNIPROT
Synonyms
C-X-C motif chemokine 2 antibody
Chemokine (C X C motif) ligand 2 antibody
Chemokine, CXC motif, ligand 2 antibody
CINC 2a antibody
CINC2a antibody
CINC3 antibody
CXC chemokine antibody
CXCL 2 antibody
Cxcl2 antibody
CXCL2_HUMAN antibody
ExpandImages
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![Sandwich ELISA analysis of Human CXCL2/MIP-2 matched pair antibodies<br /><br />Capture: <a href="/products/HA725247" style="font-weight: bold;text-decoration: underline;">HA725247</a>, Human CXCL2/MIP-2 Rabbit mAb [PSH17-88]<br />Detector: <a href="/products/HA725248" style="font-weight: bold;text-decoration: underline;">HA725248</a>, Human CXCL2/MIP-2 Rabbit mAb [PSH17-89]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA725247" style="font-weight: bold;text-decoration: underline;">HA725247</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CXCL2/MIP-2 protein (<a href="/products/HA210904" style="font-weight: bold;text-decoration: underline;">HA210904</a>) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725248" style="font-weight: bold;text-decoration: underline;">HA725248</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725248B_1.png?v=20260604174725)
Sandwich ELISA analysis of Human CXCL2/MIP-2 matched pair antibodies
Capture: HA725247, Human CXCL2/MIP-2 Rabbit mAb [PSH17-88]
Detector: HA725248, Human CXCL2/MIP-2 Rabbit mAb [PSH17-89]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725247) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CXCL2/MIP-2 protein (HA210904) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA725248, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of spiked CXCL2 in human cell culture media samples.<br /><br />Capture: <a href="/products/HA725247" style="font-weight: bold;text-decoration: underline;">HA725247</a>, Human CXCL2/MIP-2 Rabbit mAb [PSH17-88]<br />Detector: <a href="/products/HA725248" style="font-weight: bold;text-decoration: underline;">HA725248</a>, Human CXCL2/MIP-2 Rabbit mAb [PSH17-89]<br /><br />The concentrations of CXCL2 were measured in duplicates, interpolated from the CXCL2 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA725248B_2.png?v=20260604174725)
Interpolated concentrations of spiked CXCL2 in human cell culture media samples.
Capture: HA725247, Human CXCL2/MIP-2 Rabbit mAb [PSH17-88]
Detector: HA725248, Human CXCL2/MIP-2 Rabbit mAb [PSH17-89]
The concentrations of CXCL2 were measured in duplicates, interpolated from the CXCL2 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"