Biotin Conjugated Human IL-5RA Recombinant Rabbit Monoclonal Antibody [PSH24-51] - Detector

Sandwich ELISA analysis of Human IL-5RA matched pair antibodies

Capture: HA725253, Human IL-5RA Rabbit mAb [PSH24-50]
Detector: HA725254, Human IL-5RA Rabbit mAb [PSH24-51]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725253) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-5RA protein (HA210727) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA725254, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IL-5RA in human plasma samples.

Capture: HA725253, Human IL-5RA Rabbit mAb [PSH24-50]
Detector: HA725254, Human IL-5RA Rabbit mAb [PSH24-51]

The concentrations of IL-5RA were measured in duplicates, interpolated from the IL-5RA standard curve and corrected for sample dilution. Undiluted samples are human plasma 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-5RA concentration was determined to be 350.1 pg/ml in human plasma.