Biotin Conjugated Human Prealbumin Recombinant Rabbit Monoclonal Antibody [PSH17-43] - Detector

Sandwich ELISA analysis of Human Prealbumin matched pair antibodies

Capture: HA725259, Human Prealbumin Rabbit mAb [PSH17-42]
Detector: HA725260, Human Prealbumin Rabbit mAb [PSH17-43]

Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725259) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Prealbumin protein (HA211085) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA725260, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 12 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Prealbumin in human plasma.

Capture: HA725259, Human Prealbumin Rabbit mAb [PSH17-42]
Detector: HA725260, Human Prealbumin Rabbit mAb [PSH17-43]

The concentrations of Prealbumin were measured in duplicates, interpolated from the Prealbumin standard curve and corrected for sample dilution. Undiluted samples are human plasma 1:800000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean Prealbumin concentration was determined to be 357 ug/ml in human plasma.