HRP Conjugated Human CXADR Recombinant Rabbit Monoclonal Antibody [PSH17-47] - Detector
Overview
Product Name
HRP Conjugated Human CXADR Recombinant Rabbit Monoclonal Antibody [PSH17-47] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human CXADR aa 20-237 (HA211206)
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human CXADR protein (HA211206).
Conjugation
HRP
Clone Number
PSH17-47
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-46] to Human CXADR antibody (Capture) (HA725293) and Recombinant Human CXADRn protein (HA211206) as the standard. The reference range value is 625-20,000 pg/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
Component of the epithelial apical junction complex that may function as a homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with JAML a transmembrane protein of the plasma membrane of leukocytes. The interaction between both receptors also mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair.
Background References
1. Cohen C.J., Shieh J.T.C., Pickles R.J., Okegawa T., Hsieh J.-T., Bergelson J.M. The coxsackievirus and adenovirus receptor is a transmembrane component of the tight junction. Proc. Natl. Acad. Sci. U.S.A. 98:15191-15196 (2001)
2. Walters R.W., Freimuth P., Moninger T.O., Ganske I., Zabner J., Welsh M.J. Adenovirus fiber disrupts CAR-mediated intercellular adhesion allowing virus escape. Cell 110:789-799 (2002)
Subcellular Location
Cell junction, Cell membrane, Secreted.
UNIPROT
Synonyms
46 kD coxsackievirus and adenovirus receptor CAR protein antibody
46 kD coxsackievirus and adenovirus receptor protein antibody
CAR antibody
CAR4/6 antibody
Coxsackie virus and adenovirus receptor antibody
Coxsackie virus B receptor antibody
Coxsackievirus and adenovirus receptor antibody
Coxsackievirus B adenovirus receptor antibody
Coxsackievirus B-adenovirus receptor antibody
CVB3 binding protein antibody
ExpandImages
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![Sandwich ELISA analysis of Human CXADR matched pair antibodies<br /><br />Capture: <a href="/products/HA725293" style="font-weight: bold;text-decoration: underline;">HA725293</a>, Human CXADR Rabbit mAb [PSH17-46]<br />Detector: <a href="/products/HA725294" style="font-weight: bold;text-decoration: underline;">HA725294</a>, Human CXADR Rabbit mAb [PSH17-47]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725293" style="font-weight: bold;text-decoration: underline;">HA725293</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CXADR protein (<a href="/products/HA211206" style="font-weight: bold;text-decoration: underline;">HA211206</a>) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725294" style="font-weight: bold;text-decoration: underline;">HA725294</a>, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725294H_1.jpg?v=20260401003221)
Sandwich ELISA analysis of Human CXADR matched pair antibodies
Capture: HA725293, Human CXADR Rabbit mAb [PSH17-46]
Detector: HA725294, Human CXADR Rabbit mAb [PSH17-47]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725293) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CXADR protein (HA211206) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA725294, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"