HRP Conjugated Human Lumican Recombinant Rabbit Monoclonal Antibody [PSH19-05] - Detector
Overview
Product Name
HRP Conjugated Human Lumican Recombinant Rabbit Monoclonal Antibody [PSH19-05] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Recombinant Human Lumican aa 19-338 (HA211171).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human Lumican protein (HA211171).
Conjugation
HRP
Clone Number
PSH19-05
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-04] to Human Lumican antibody (Capture) (HA725317) and Recombinant Human Lumican protein (HA211171) as the standard. The reference range value is 78.1-80,000 pg/mL.
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ELISA
-
Use at an assay dependent concentration.
Target
Function
Lumican, also known as LUM, is an extracellular matrix protein that, in humans, is encoded by the LUM gene on chromosome 12. Lumican is a major keratan sulfate proteoglycan of the cornea but is ubiquitously distributed in most mesenchymal tissues throughout the body. Lumican is involved in collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. Corneal transparency is possible due to the exact alignment of collagen fibers by lumican (and keratocan) in the intrafibrillar space.
Background References
1. Smith MM et al. Lumican, a Multifunctional Cell Instructive Biomarker Proteoglycan Has Novel Roles as a Marker of the Hypercoagulative State of Long Covid Disease. Int J Mol Sci. 2024 Feb
2. Cho HJ et al. Lumican, an Exerkine, Protects against Skeletal Muscle Loss. Int J Mol Sci. 2022 Sep
Subcellular Location
Secreted, extracellular space, extracellular matrix.
UNIPROT
Synonyms
Keratan sulfate proteoglycan lumican antibody
KSPG lumican antibody
LDC antibody
LUM antibody
LUM_HUMAN antibody
Lumican antibody
Lumican precursor antibody
SLRR2D antibody
Images
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![Sandwich ELISA analysis of Human Lumican matched pair antibodies<br /><br />Capture: <a href="/products/HA725317" style="font-weight: bold;text-decoration: underline;">HA725317</a>, Human Lumican Rabbit mAb [PSH19-04]<br />Detector: <a href="/products/HA725318" style="font-weight: bold;text-decoration: underline;">HA725318</a>, Human Lumican Rabbit mAb [PSH19-05]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725317" style="font-weight: bold;text-decoration: underline;">HA725317</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Lumican protein (<a href="/products/HA211171" style="font-weight: bold;text-decoration: underline;">HA211171</a>) starting from 80,000pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725318" style="font-weight: bold;text-decoration: underline;">HA725318</a>, HRP, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725318H_1.jpg?v=20260604174654)
Sandwich ELISA analysis of Human Lumican matched pair antibodies
Capture: HA725317, Human Lumican Rabbit mAb [PSH19-04]
Detector: HA725318, Human Lumican Rabbit mAb [PSH19-05]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725317) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Lumican protein (HA211171) starting from 80,000pg/ml to 0 pg/ml and detect antibody (HA725318, HRP, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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