Human NCR1/NKp46 Recombinant Rabbit Monoclonal Antibody [PSH17-32] - BSA and Azide free (Detector)

Sandwich ELISA analysis of Human NCR1/NKp46 matched pair antibodies

Capture: HA725321, Human NCR1/NKp46 Rabbit mAb [PSH17-31]
Detector: HA725322, Human NCR1/NKp46 Rabbit mAb [PSH17-32]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725321) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human NCR1/NKp46 protein (HA211272)starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA725322, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native NCR1 in NK-92 cell culture supernatant.

Capture: HA725321, Human NCR1/NKp46 Rabbit mAb [PSH17-31]
Detector: HA725322, Human NCR1/NKp46 Rabbit mAb [PSH17-32]

Interpolated concentration of native NCR1 was measured in duplicate at different sample concentrations and interpolated from the NCR1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean spp1 concentration was determined to be 533 pg/ml in NK-92 cell culture supernatant.