Human IL-32 Recombinant Rabbit Monoclonal Antibody [PSH20-78] - BSA and Azide free (Capture)
Overview
Product Name
Human IL-32 Recombinant Rabbit Monoclonal Antibody [PSH20-78] - BSA and Azide free (Capture)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Recombinant Human IL-32 aa 1-131 (HA211338).
Species Reactivity
Human
Validated Applications
ELISA(Cap)
Positive Control
Recombinant Human IL-32 protein (HA211338).
Conjugation
unconjugated
Clone Number
PSH20-78
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-79] to Human IL-32 antibody (Detector) (HA725370) and Recombinant Human IL-32 protein (HA211338) as the standard. The reference range value is 31.3-8,000 pg/mL.
Target
Function
Interleukin 32 (IL32) is proinflammatory cytokine that in humans is encoded by the IL32 gene. Interleukin 32 can be found in higher mammals but not in rodents. It is mainly expressed intracellularly and the protein has nine different isoforms, because the pre-mRNA can be alternatively spliced. The most active and studied isoform is IL-32γ. It was first reported in 2005, although the IL-32 gene was first described in 1992. It does not belong to any cytokine family because there is almost no homology with other cytokines. This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNF-alpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Interleukin 32 (IL-32) is a pro-inflammatory cytokine that can induce cells of the immune system (such as monocytes and macrophages) to secrete inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α) and IL-6. In addition, it can also induce the production of chemokines such as IL-8 and MIP-2 / CXCL2. IL-32 can also support osteoclast differentiation but not osteoclast activation by regulating the MAPK/ERK pathway and the actin cytoskeleton.
Background References
1. Gautam A et al. IL32: The multifaceted and unconventional cytokine. Hum Immunol. 2021 Sep
2. Sasidharan K et al. IL32 downregulation lowers triglycerides and type I collagen in di-lineage human primary liver organoids. Cell Rep Med. 2024 Jan
Subcellular Location
Secreted.
UNIPROT
Synonyms
IL 32alpha antibody
IL 32beta antibody
IL 32delta antibody
IL 32gamma antibody
IL-32 antibody
IL32 antibody
IL32_HUMAN antibody
Interleukin 32 antibody
Interleukin 32 small antibody
Interleukin 32 theta antibody
ExpandImages
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![Sandwich ELISA analysis of Human IL-32 matched pair antibodies<br /><br />Capture: <a href="/products/HA725369" style="font-weight: bold;text-decoration: underline;">HA725369</a>, Human IL-32 Rabbit mAb [PSH20-78]<br />Detector: <a href="/products/HA725370" style="font-weight: bold;text-decoration: underline;">HA725370</a>, Human IL-32 Rabbit mAb [PSH20-79]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA725369" style="font-weight: bold;text-decoration: underline;">HA725369</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-32 protein (<a href="/products/HA211338" style="font-weight: bold;text-decoration: underline;">HA211338</a>) starting from 7,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725370" style="font-weight: bold;text-decoration: underline;">HA725370</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725369_1.png?v=20260429162349)
Sandwich ELISA analysis of Human IL-32 matched pair antibodies
Capture: HA725369, Human IL-32 Rabbit mAb [PSH20-78]
Detector: HA725370, Human IL-32 Rabbit mAb [PSH20-79]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725369) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-32 protein (HA211338) starting from 7,000 pg/ml to 0 pg/ml and detect antibody (HA725370, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native IL-32 in NK-92 samples.<br /><br />Capture: <a href="/products/HA725369" style="font-weight: bold;text-decoration: underline;">HA725369</a>, Human IL-32 Rabbit mAb [PSH20-78]<br />Detector: <a href="/products/HA725370" style="font-weight: bold;text-decoration: underline;">HA725370</a>, Human IL-32 Rabbit mAb [PSH20-79]<br /><br />The concentrations of IL-32 were measured in duplicates, interpolated from the IL-32 standard curve and corrected for sample dilution. Undiluted samples are NK-92. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-32 concentration was determined to be 17,271 pg/ml in NK-92.](https://storage.huabio.cn/huabio/productImg/HA725369_2.png?v=20260429162349)
Interpolated concentrations of native IL-32 in NK-92 samples.
Capture: HA725369, Human IL-32 Rabbit mAb [PSH20-78]
Detector: HA725370, Human IL-32 Rabbit mAb [PSH20-79]
The concentrations of IL-32 were measured in duplicates, interpolated from the IL-32 standard curve and corrected for sample dilution. Undiluted samples are NK-92. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-32 concentration was determined to be 17,271 pg/ml in NK-92.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
IL-32 Rabbit Polyclonal Antibody
Application: WB,IF-Cell,IHC-P,FC,IF-Tissue
Reactivity: Human
Conjugate: unconjugated
Human IL-32 Recombinant Rabbit Monoclonal Antibody [PSH20-79] - BSA and Azide free (Detector)
Application: ELISA(Det)
Reactivity: Human
Conjugate: unconjugated
