Biotin Conjugated Human PLGF Recombinant Rabbit Monoclonal Antibody [PSH23-86] - Detector
Overview
Product Name
Biotin Conjugated Human PLGF Recombinant Rabbit Monoclonal Antibody [PSH23-86] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human PLGF aa 19-149 (HA211337).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human PLGF protein (HA211337).
Conjugation
Biotin
Clone Number
PSH23-86
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH23-85] to Human PLGF (Capture) (HA725377) and Recombinant Human PLGF protein (HA211337) as the standard. The reference range value is 39.1-10,000 pg/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
Placental growth factor (PlGF) is a protein that in humans is encoded by the PGF gene. Placental growth factor is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. The main source of PlGF during pregnancy is the placental trophoblast. PGF is also expressed in many other tissues, including the villous trophoblast. The placental growth factor gene (PGF) is a protein-coding gene and a member of the vascular endothelial growth factor (VEGF) family. PGF is expressed only in human umbilical vein endothelial cells, and the placenta. PGF is ultimately associated with angiogenesis. Specifically, PGF plays a role in trophoblast growth and differentiation. Trophoblast cells, specifically extravillous trophoblast cells, are responsible for invading the uterine wall and the maternal spiral arteries. The extravillous trophoblast cells produce a blood vessel of larger diameter for the developing fetus that is independent of maternal vasoconstriction. This is essential for increased blood flow and reduced resistance. Proper development of blood vessels in the placenta is crucial for the higher blood requirement of the fetus later in pregnancy. Under normal physiologic conditions, PGF is also expressed at a low level in other organs including the heart, lung, thyroid, and skeletal muscle.
Background References
1. Stepan H et al. Clinical utility of sFlt-1 and PlGF in screening, prediction, diagnosis and monitoring of pre-eclampsia and fetal growth restriction. Ultrasound Obstet Gynecol. 2023 Feb
2. Verlohren S et al. Clinical interpretation and implementation of the sFlt-1/PlGF ratio in the prediction, diagnosis and management of preeclampsia. Pregnancy Hypertens. 2022 Mar
Subcellular Location
Secreted.
UNIPROT
Synonyms
D12S1900 antibody
Pgf antibody
PGFL antibody
PIGF antibody
Placenta growth factor antibody
Placental growth factor antibody
Placental growth factor, vascular endothelial growth factor related protein antibody
PlGF 2 antibody
PlGF antibody
PLGF_HUMAN antibody
ExpandImages
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![Sandwich ELISA analysis of Human PLGF matched pair antibodies<br /><br />Capture: <a href="/products/HA725377" style="font-weight: bold;text-decoration: underline;">HA725377</a>, Human PLGF Rabbit mAb [PSH23-85]<br />Detector: <a href="/products/HA725378" style="font-weight: bold;text-decoration: underline;">HA725378</a>, Human PLGF Rabbit mAb [PSH23-86]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725377" style="font-weight: bold;text-decoration: underline;">HA725377</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human PLGF protein (<a href="/products/HA211337" style="font-weight: bold;text-decoration: underline;">HA211337</a>) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725378" style="font-weight: bold;text-decoration: underline;">HA725378</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725378B_1.png?v=20260604174644)
Sandwich ELISA analysis of Human PLGF matched pair antibodies
Capture: HA725377, Human PLGF Rabbit mAb [PSH23-85]
Detector: HA725378, Human PLGF Rabbit mAb [PSH23-86]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725377) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human PLGF protein (HA211337) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725378, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native PLGF in JAR cell culture supernatant.<br /><br />Capture: <a href="/products/HA725377" style="font-weight: bold;text-decoration: underline;">HA725377</a>, Human PLGF Rabbit mAb [PSH23-85]<br />Detector: <a href="/products/HA725378" style="font-weight: bold;text-decoration: underline;">HA725378</a>, Human PLGF Rabbit mAb [PSH23-86]<br /><br />The concentrations of PLGF were measured in duplicates, interpolated from the PLGF standard curve and corrected for sample dilution. Undiluted samples are 100% JAR cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PLGF concentration was determined to be 1,457 pg/ml in JAR cell culture supernatant.](https://storage.huabio.cn/huabio/productImg/HA725378B_2.png?v=20260604174644)
Interpolated concentrations of native PLGF in JAR cell culture supernatant.
Capture: HA725377, Human PLGF Rabbit mAb [PSH23-85]
Detector: HA725378, Human PLGF Rabbit mAb [PSH23-86]
The concentrations of PLGF were measured in duplicates, interpolated from the PLGF standard curve and corrected for sample dilution. Undiluted samples are 100% JAR cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PLGF concentration was determined to be 1,457 pg/ml in JAR cell culture supernatant.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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