Biotin Conjugated Human POMC Recombinant Rabbit Monoclonal Antibody [PSH22-66] - Detector
Overview
Product Name
Biotin Conjugated Human POMC Recombinant Rabbit Monoclonal Antibody [PSH22-66] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human POMC aa 26-267 (HA211433).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human POMC protein (HA211433).
Conjugation
Biotin
Clone Number
PSH22-66
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH22-65] to Human POMC antibody (Capture) (HA725395) and Recombinant Human POMC protein (HA211433) as the standard. The reference range value is 15.6-1,000 pg/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
Pro-opiomelanocortin (POMC) is a precursor polypeptide with 241 amino acid residues. POMC is synthesized in corticotrophs of the anterior pituitary from the 267-amino-acid-long polypeptide precursor pre-pro-opiomelanocortin (pre-POMC), by the removal of a 26-amino-acid-long signal peptide sequence during translation. POMC is part of the central melanocortin system. This gene encodes a preproprotein that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the preproprotein and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. The antimicrobial melanotropin alpha peptide exhibits antibacterial and antifungal activity. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.
Background References
1. Quarta C et al. POMC neuronal heterogeneity in energy balance and beyond: an integrated view. Nat Metab. 2021 Mar
2. Clément K et al. Efficacy and safety of setmelanotide, an MC4R agonist, in individuals with severe obesity due to LEPR or POMC deficiency: single-arm, open-label, multicentre, phase 3 trials. Lancet Diabetes Endocrinol. 2020 Dec
Subcellular Location
Secreted.
UNIPROT
Synonyms
ACTH antibody
Adrenocorticotropic hormone antibody
Adrenocorticotropin antibody
alpha melanocyte stimulating hormone antibody
Alpha MSH antibody
Alpha-MSH antibody
beta endorphin antibody
Beta LPH antibody
beta melanocyte stimulating hormone antibody
Beta MSH antibody
ExpandImages
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![Sandwich ELISA analysis of Human POMC matched pair antibodies<br /><br />Capture: <a href="/products/HA725395" style="font-weight: bold;text-decoration: underline;">HA725395</a>, Human POMC Rabbit mAb [PSH22-65]<br />Detector: <a href="/products/HA725396" style="font-weight: bold;text-decoration: underline;">HA725396</a>, Human POMC Rabbit mAb [PSH22-66]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA725395" style="font-weight: bold;text-decoration: underline;">HA725395</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted ACTH protein starting from 2,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725396" style="font-weight: bold;text-decoration: underline;">HA725396</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725396B_1.png?v=20260604174653)
Sandwich ELISA analysis of Human POMC matched pair antibodies
Capture: HA725395, Human POMC Rabbit mAb [PSH22-65]
Detector: HA725396, Human POMC Rabbit mAb [PSH22-66]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725395) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted ACTH protein starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725396, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Human POMC Recombinant Rabbit Monoclonal Antibody [PSH22-65] - BSA and Azide free (Capture)
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Human POMC Recombinant Rabbit Monoclonal Antibody [PSH22-66] - BSA and Azide free (Detector)
Application: ELISA(Det)
Reactivity: Human
Conjugate: unconjugated
POMC Recombinant Rabbit Monoclonal Antibody [JE32-88]
Application: WB,IHC-P,IF-Tissue
Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig
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