Human TNFSF14 Recombinant Rabbit Monoclonal Antibody [PSH27-26] - BSA and Azide free (Capture)
Overview
Product Name
Human TNFSF14 Recombinant Rabbit Monoclonal Antibody [PSH27-26] - BSA and Azide free (Capture)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human TNFSF14 aa 74-240 (HA211392).
Species Reactivity
Human
Validated Applications
ELISA(Cap)
Positive Control
Recombinant Human TNFSF14 protein (HA211392).
Conjugation
unconjugated
Clone Number
PSH27-26
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH27-27] to Human TNFSF14 (Detecor) (HA725534) and Recombinant Human TNFSF14 protein (HA211392) as the standard. The reference range value is 3.9-1,000 pg/mL.
Target
Function
LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3. The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). Two alternatively spliced transcript variant encoding distinct isoforms have been reported. This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, trigger apoptosis of various tumor cells and play a role in vascular normalisation processes. This protein is also reported to prevent tumor necrosis factor alpha-mediated apoptosis in primary hepatocytes. Similar to how CD4 is the primary mediating receptor in HIV infection, the HSV glycoprotein (gD) binds to the HVEM receptor which is demanded by TNFSF14/LIGHT lowering the ability for LIGHT to activate the NFκB pathway. NFκB is a survival factor helping to inhibit apoptosis which triggers a pathway inhibiting caspase 8. When gD from HSV binds to HVEM, LIGHT is non-competitively inhibited from binding, encouraging apoptosis in the infected cell.
Background References
1. Mousa RS et al. TNFSF14 (LIGHT) in intestinal inflammation: balancing immune activation and resolution in IBD. Front Immunol. 2025 Sep
2. Wu Y et al. TNFSF14/LIGHT promotes cardiac fibrosis and atrial fibrillation vulnerability via PI3Kgamma/SGK1 pathway-dependent M2 macrophage polarisation. J Transl Med. 2023 Aug
Subcellular Location
Cell membrane, Secreted, Cytoplasm.
UNIPROT
Synonyms
CD 258 antibody
CD258 antibody
CD258 antigen antibody
Delta transmembrane LIGHT antibody
Herpes virus entry mediator ligand antibody
Herpesvirus entry mediator A antibody
Herpesvirus entry mediator ligand antibody
herpesvirus entry mediator-ligand antibody
HVEM L antibody
HVEM-L antibody
ExpandImages
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![Sandwich ELISA analysis of Human TNFSF14 matched pair antibodies<br /><br />Capture: <a href="/products/HA725533" style="font-weight: bold;text-decoration: underline;">HA725533</a>, Human TNFSF14 Rabbit mAb [PSH27-26]<br />Detector: <a href="/products/HA725534" style="font-weight: bold;text-decoration: underline;">HA725534</a>, Human TNFSF14 Rabbit mAb [PSH27-27]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725533" style="font-weight: bold;text-decoration: underline;">HA725533</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TNFSF14 protein (<a href="/products/HA211392" style="font-weight: bold;text-decoration: underline;">HA211392</a>) starting from 1,000 pg/mL to 0 pg/mL and detect antibody (<a href="/products/HA725534" style="font-weight: bold;text-decoration: underline;">HA725534</a>, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725533_1.png?v=20260818230509)
Sandwich ELISA analysis of Human TNFSF14 matched pair antibodies
Capture: HA725533, Human TNFSF14 Rabbit mAb [PSH27-26]
Detector: HA725534, Human TNFSF14 Rabbit mAb [PSH27-27]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725533) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TNFSF14 protein (HA211392) starting from 1,000 pg/mL to 0 pg/mL and detect antibody (HA725534, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native TNFSF14 in KG-1 and MCF7 cell extract samples based on 1 mg/mL extract loading.<br /><br />Capture: <a href="/products/HA725533" style="font-weight: bold;text-decoration: underline;">HA725533</a>, Human TNFSF14 Rabbit mAb [PSH27-26]<br />Detector: <a href="/products/HA725534" style="font-weight: bold;text-decoration: underline;">HA725534</a>, Human TNFSF14 Rabbit mAb [PSH27-27]<br /><br />The concentrations of TNFSF14 were measured in duplicates, interpolated from the TNFSF14 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNFSF14 concentration was determined to be 385 pg/mL in KG-1 cell extract. There was no detectable signal in MCF7 cell extract.](https://storage.huabio.cn/huabio/productImg/HA725533_2.png?v=20260818230509)
Interpolated concentrations of native TNFSF14 in KG-1 and MCF7 cell extract samples based on 1 mg/mL extract loading.
Capture: HA725533, Human TNFSF14 Rabbit mAb [PSH27-26]
Detector: HA725534, Human TNFSF14 Rabbit mAb [PSH27-27]
The concentrations of TNFSF14 were measured in duplicates, interpolated from the TNFSF14 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNFSF14 concentration was determined to be 385 pg/mL in KG-1 cell extract. There was no detectable signal in MCF7 cell extract.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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