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Lamin B1 Recombinant Rabbit Monoclonal Antibody [SI17-07] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750098

Lamin B1 Recombinant Rabbit Monoclonal Antibody [SI17-07] - BSA and Azide free

Application

  • WB

  • IF-Tissue

  • IHC-P

  • CUT&Tag-seq

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Lamin B1 Recombinant Rabbit Monoclonal Antibody [SI17-07] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Lamin B1 aa 511-560 / 586.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Tissue, IHC-P, CUT&Tag-seq

Molecular Weight

Predicted band size: 66 kDa

Positive Control

HeLa cell lysate, HEK-293 cell lysate, THP-1 cell lysate, SH-SY5Y cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, human liver tissue, human breast carcinoma tissue, human colon carcinoma tissue, human lymph nodes tissue, mouse large intestine tissue, rat liver tissue.

Conjugation

unconjugated

Clone Number

SI17-07

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:50,000

  • IF-Tissue

  • 1:500-1:1,000

  • IHC-P

  • 1:500-1:1,000

Target

Function

A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, termed Ced-3/ICE, functions as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Nuclear lamins are critical to maintaining the integrity of the nuclear envelope and cellular morphology as components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family.

Background References

1. Cruz OH et al. Multinucleation and Polykaryon Formation is Promoted by the EhPC4 Transcription Factor in Entamoeba histolytica. Sci Rep 6:19611 (2016).

2. Lund K et al. Slug-dependent upregulation of L1CAM is responsible for the increased invasion potential of pancreatic cancer cells following long-term 5-FU treatment. PLoS One 10:e0123684 (2015).

Sequence Similarity

Belongs to the intermediate filament family.

Post-translational Modification

B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.

Subcellular Location

Nucleus inner membrane.

UNIPROT

SwissProt: P20700 Human

SwissProt: P14733 Mouse

SwissProt: P70615 Rat

Synonyms

ADLD antibody

lamin B1 antibody

Lamin-B1 antibody

LMN antibody

LMN2 antibody

LMNB antibody

Lmnb1 antibody

LMNB1_HUMAN antibody

MGC111419 antibody

OTTHUMP00000159218 antibody

Images

  • Western blot analysis of Lamin B1 on different lysates with Rabbit anti-Lamin B1 antibody (<a href="/products/HA750098" style="font-weight: bold;text-decoration: underline;">HA750098</a>) at 1/50,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HEK-293 cell lysate<br />Lane 3: THP-1 cell lysate<br />Lane 4: SH-SY5Y cell lysate<br />Lane 5: C2C12 cell lysate<br />Lane 6: NIH/3T3 cell lysate<br />Lane 7: C6 cell lysate<br />Lane 8: PC-12 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 66 kDa<br />Observed band size: 66 kDa<br /><br />Exposure time: 21 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750098" style="font-weight: bold;text-decoration: underline;">HA750098</a>) at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Lamin B1 on different lysates with Rabbit anti-Lamin B1 antibody (HA750098) at 1/50,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HEK-293 cell lysate
    Lane 3: THP-1 cell lysate
    Lane 4: SH-SY5Y cell lysate
    Lane 5: C2C12 cell lysate
    Lane 6: NIH/3T3 cell lysate
    Lane 7: C6 cell lysate
    Lane 8: PC-12 cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 66 kDa
    Observed band size: 66 kDa

    Exposure time: 21 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750098) at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockout (KO)</span><br /><br />All lanes: Western blot analysis of Lamin B1 with anti-Lamin B1 antibody [SI17-06] (<a href="/products/HA750098" style="font-weight: bold;text-decoration: underline;">HA750098</a>) at 1:1,000 dilution.<br />Lane 1/2: Wild-type Hela whole cell lysate (20 µg).<br />Lane 3/4: Lamin B1 knockout Hela whole cell lysate (20 µg).<br /><br /><a href="/products/ET1606-27" style="font-weight: bold;text-decoration: underline;">ET1606-27</a> was shown to specifically react with Lamin B1 in wild-type Hela cells. No band was observed when Lamin B1 knockout samples were tested. Wild-type and Lamin B1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1606-27" style="font-weight: bold;text-decoration: underline;">ET1606-27</a>, 1/1,000) and Loading control antibody(Rabbit anti-Vinculin, <a href="/products/ET1705-94" style="font-weight: bold;text-decoration: underline;">ET1705-94</a>, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    ☑ Knockout (KO)

    All lanes: Western blot analysis of Lamin B1 with anti-Lamin B1 antibody [SI17-06] (HA750098) at 1:1,000 dilution.
    Lane 1/2: Wild-type Hela whole cell lysate (20 µg).
    Lane 3/4: Lamin B1 knockout Hela whole cell lysate (20 µg).

    ET1606-27 was shown to specifically react with Lamin B1 in wild-type Hela cells. No band was observed when Lamin B1 knockout samples were tested. Wild-type and Lamin B1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1606-27, 1/1,000) and Loading control antibody(Rabbit anti-Vinculin, ET1705-94, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Lamin B1 antibody (<a href="/products/HA750098" style="font-weight: bold;text-decoration: underline;">HA750098</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750098" style="font-weight: bold;text-decoration: underline;">HA750098</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Lamin B1 antibody (HA750098) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750098) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Lamin B1 antibody (<a href="/products/HA750098" style="font-weight: bold;text-decoration: underline;">HA750098</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750098" style="font-weight: bold;text-decoration: underline;">HA750098</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Lamin B1 antibody (HA750098) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750098) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Lamin B1 antibody (<a href="/products/HA750098" style="font-weight: bold;text-decoration: underline;">HA750098</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750098" style="font-weight: bold;text-decoration: underline;">HA750098</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Lamin B1 antibody (HA750098) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750098) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Lamin B1 antibody (<a href="/products/HA750098" style="font-weight: bold;text-decoration: underline;">HA750098</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750098" style="font-weight: bold;text-decoration: underline;">HA750098</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Lamin B1 antibody (HA750098) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750098) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-Lamin B1 antibody (<a href="/products/HA750098" style="font-weight: bold;text-decoration: underline;">HA750098</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750098" style="font-weight: bold;text-decoration: underline;">HA750098</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-Lamin B1 antibody (HA750098) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750098) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Lamin B1 antibody (<a href="/products/HA750098" style="font-weight: bold;text-decoration: underline;">HA750098</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750098" style="font-weight: bold;text-decoration: underline;">HA750098</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Lamin B1 antibody (HA750098) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750098) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: breast carcinoma<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/1,000

    Application: IF-Tissue

    Species: Human

    Site: breast carcinoma

    Sample: Paraffin-embedded section

    Antibody concentration: 1/1,000

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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