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GST Recombinant Rabbit Monoclonal Antibody [SN70-05] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750257

GST Recombinant Rabbit Monoclonal Antibody [SN70-05] - BSA and Azide free

Application

  • WB

  • IF-Cell

  • IP

Reactivity

  • Species independent

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

GST Recombinant Rabbit Monoclonal Antibody [SN70-05] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

GST tag recombinant protein.

Species Reactivity

Species independent

Validated Applications

WB, IF-Cell, IP

Positive Control

293T transfected with GST cell lysate, 293T transfected with GST-tagged Histone H3.1 cell lysate, HeLa transfected with GST-tagged Histone H3.1 cells.

Conjugation

unconjugated

Clone Number

SN70-05

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:50,000

  • IF-Cell

  • 1:50-1:200

  • IP

  • Use at an assay dependent concentration.

Target

Function

The glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. Mammalian cytosolic GSTs are dimeric both subunits being from the same class of GSTs. The monomers are in the range of 22–29 kDa. Glutathione S-transferase is used to create the “GST gene fusion system” in genetic engineering. Here, GST is used to purify and detect proteins of interest.

Background References

1. Pettit MW et al. Construction and physiochemical characterisation of a multi-composite, potential oral vaccine delivery system (VDS). Int J Pharm 468:264-71 (2014).

2. Zhang D et al. The Chromatin-Remodeling Factor PICKLE Integrates Brassinosteroid and Gibberellin Signaling during Skotomorphogenic Growth in Arabidopsis. Plant Cell 26:2472-2485 (2014).

Synonyms

Glutathione S Transferase antibody

Glutathione S-transferase class-mu 26 kDa isozyme antibody

GST antibody

Sj26 antigen antibody

SjGST antibody

Images

  • Western blot analysis of GST on different lysates with Rabbit anti-GST antibody (<a href="/products/HA750257" style="font-weight: bold;text-decoration: underline;">HA750257</a>) at 1/50,000 dilution.<br /><br />Lane 1: 293T transfected with GST cell lysate (2.5 µg/Lane)<br />Lane 2: 293T transfected with GST-tagged Histone H3.1 cell lysate (15 µg/Lane)<br /><br />Exposure time: 46 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750257" style="font-weight: bold;text-decoration: underline;">HA750257</a>) at 1/50,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GST on different lysates with Rabbit anti-GST antibody (HA750257) at 1/50,000 dilution.

    Lane 1: 293T transfected with GST cell lysate (2.5 µg/Lane)
    Lane 2: 293T transfected with GST-tagged Histone H3.1 cell lysate (15 µg/Lane)

    Exposure time: 46 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750257) at 1/50,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • GST was immunoprecipitated in 0.2mg 293T-OE-GST cell lysate with <a href="/products/HA750257" style="font-weight: bold;text-decoration: underline;">HA750257</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA750257" style="font-weight: bold;text-decoration: underline;">HA750257</a> at 1/50,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: 293T transfected with GST-tagged Histone H3.1 cell lysate (input)<br />Lane 2: Rabbit IgG instead of <a href="/products/HA750257" style="font-weight: bold;text-decoration: underline;">HA750257</a> in 293T transfected with GST-tagged Histone H3.1 cell lysate<br />Lane 3: <a href="/products/HA750257" style="font-weight: bold;text-decoration: underline;">HA750257</a> IP in 293T transfected with GST-tagged Histone H3.1 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 24 seconds

    GST was immunoprecipitated in 0.2mg 293T-OE-GST cell lysate with HA750257 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750257 at 1/50,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: 293T transfected with GST-tagged Histone H3.1 cell lysate (input)
    Lane 2: Rabbit IgG instead of HA750257 in 293T transfected with GST-tagged Histone H3.1 cell lysate
    Lane 3: HA750257 IP in 293T transfected with GST-tagged Histone H3.1 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 24 seconds

  • Immunocytochemistry analysis of HeLa transfected with GST-tagged Histone H3.1 cells labeling GST with Rabbit anti-GST antibody (<a href="/products/HA750257" style="font-weight: bold;text-decoration: underline;">HA750257</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GST antibody (<a href="/products/HA750257" style="font-weight: bold;text-decoration: underline;">HA750257</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Histone H3 (<a href="/products/HA601278" style="font-weight: bold;text-decoration: underline;">HA601278</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa transfected with GST-tagged Histone H3.1 cells labeling GST with Rabbit anti-GST antibody (HA750257) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GST antibody (HA750257) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Histone H3 (HA601278, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"