GST Recombinant Rabbit Monoclonal Antibody [SN70-05] (ET1611-47)
Catalog# ET1611-47
Rabbit Monoclonal to GST
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WB
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IF-Cell
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IP
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Species independent
Lane 1: 293T transfected with GST cell lysate (2.5 µg/Lane)
Lane 2: 293T transfected with GST-tagged Histone H3.1 cell lysate (15 µg/Lane)
Exposure time: 46 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-47) at 1/50,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
GST Recombinant Rabbit Monoclonal Antibody [SN70-05] (ET1611-47)
Immunogen
Gst tag recombinant protein.
Host
Rabbit
Positive Control
293T transfected with GST cell lysate, 293T transfected with GST-tagged Histone H3.1 cell lysate, HeLa transfected with GST-tagged Histone H3.1 cells.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN70-05
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:50,000
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IF-Cell
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1:50-1:200
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Isotype
IgG
TARGET
PROTEIN NAME
GST
SYNONYMS
Glutathione S Transferase antibody;Glutathione S-transferase class-mu 26 kDa isozyme antibody;GST antibody;Sj26 antigen antibody;SjGST antibody
FUNCTION
The glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. Mammalian cytosolic GSTs are dimeric both subunits being from the same class of GSTs. The monomers are in the range of 22–29 kDa. Glutathione S-transferase is used to create the “GST gene fusion system” in genetic engineering. Here, GST is used to purify and detect proteins of interest.
CITATIONS
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The LAMMER Kinase MoKns1 Regulates Growth, Conidiation and Pathogenicity in Magnaporthe oryzae
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IP
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Magnaporthe oryzae
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Citation
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Guoqiang Xu;Xiangao Huang
Caspase-8 Inhibition Prevents the Cleavage and Degradation of E3 Ligase Substrate Receptor Cereblon and Potentiates Its Biological Function
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IP
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human
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Citation
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