CD10 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [SN75-07] (HA750273)
Catalog# HA750273
Rabbit Monoclonal to CD10
Tested for
-
WB
-
FC
-
IHC-P
-
IF-Cell
-
IF-Tissue
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of CD10 on different lysates with Rabbit anti-CD10 antibody (HA750273) at 1/2,000 dilution.
Lane 1: Ramos cell lysate (15 µg/Lane)
Lane 2: Daudi cell lysate (15 µg/Lane)
Lane 3: U-2 OS cell lysate (negative) (15 µg/Lane)
Lane 4: Mouse kidney tissue lysate (20 µg/Lane)
Lane 5: Mouse small intestine tissue lysate (20 µg/Lane)
Lane 6: Rat lung tissue lysate (20 µg/Lane)
Predicted band size: 86 kDa
Observed band size: 100 kDa
Exposure time: 24 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750273) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CD10 on different lysates with Rabbit anti-CD10 antibody (HA750273) at 1/2,000 dilution.
Lane 1: Ramos cell lysate (15 µg/Lane)
Lane 2: Daudi cell lysate (15 µg/Lane)
Lane 3: U-2 OS cell lysate (negative) (15 µg/Lane)
Lane 4: Mouse kidney tissue lysate (20 µg/Lane)
Lane 5: Mouse small intestine tissue lysate (20 µg/Lane)
Lane 6: Rat lung tissue lysate (20 µg/Lane)
Predicted band size: 86 kDa
Observed band size: 100 kDa
Exposure time: 24 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750273) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.