Cytokeratin 9 Recombinant Rabbit Monoclonal Antibody [SD201-09] - BSA and Azide free
Catalog# HA750302
Cytokeratin 9 Recombinant Rabbit Monoclonal Antibody [SD201-09] - BSA and Azide free
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WB
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IHC-P
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IF-Tissue
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IF-Cell
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IP
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Human
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Mouse
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ET1612-77
含抗保成分
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Rat
Predicted species support after-sales service
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unconjugated
Overview
Product Name
Cytokeratin 9 Recombinant Rabbit Monoclonal Antibody [SD201-09] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein wiehin Human Cytokeratin 9 aa 234-422 / 623.
Species Reactivity
Human, Mouse (Predicted: Rat)
Predicted species support after-sales service
Validated Applications
WB, IHC-P, IF-Tissue, IF-Cell, IP
Molecular Weight
Predicted band size: 62 kDa
Positive Control
HAP-1 cell lysates, mouse skin tissue, human breast tissue, MCF-7.
Conjugation
unconjugated
Clone Number
SD201-09
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IF-Cell
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1:50
Target
Function
Cytokeratins comprise a diverse group of intermediate filament proteins that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratin 9 is an unusually large, type I acidic cytokeratin that differentiates human plantar and palmar epidermal cells. Cytokeratin 9 localizes to the suprabasal layers as well as the upper epidermal layers such as the glandular ridges and interridges. The domains of cytokeratin 9 include a head, an α-helical coiled-coil-forming rod, and a tail; cytokeratin 9 shares significant homology with cytokeratin 10. Mutations in the cytokeratin 9 gene correlate with the development of epidermolytic palmoplantar keratoderma (EPPK), an autosomal dominant inherited skin disorder that is characterized by hyperkeratosis of the skin over the palms and soles.
Background References
1. De Rosa, L. et al. 2014. Long-term stability and safety of transgenic cultured epidermal stem cells in gene therapy of junctional epidermolysis bullosa. Stem Cell Reports. 2: 1-8.
2. Rice RH et al. 2013. Distinguishing ichthyoses by protein profiling. PLoS One 8:e75355.
Sequence Similarity
Belongs to the intermediate filament family.
Tissue Specificity
Expressed in the terminally differentiated epidermis of palms and soles.
Subcellular Location
Extracellular exosome, membrane, nucleus.
Synonyms
CK 9 antibody
CK9 antibody
CK-9 antibody
Cytokeratin 9 antibody
Cytokeratin-9 antibody
Cytokeratin9 antibody
EPPK antibody
K1C9_HUMAN antibody
K9 antibody
Keratin 9 antibody
ExpandImages
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☑ Knockdown (KD)
Western blot analysis of Cytokeratin 9 on different lysates with Rabbit anti-Cytokeratin 9 antibody (HA750302) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Cytokeratin 9 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 18 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750302) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750302, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 9 (HA750302) and Vimentin (EM0401).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 9 (ET1612-77, red) at 1/50 dilution and Vimentin (EM0401, green) at 1/500 dilution at +4℃ overnight, washed with PBS.
Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) and Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 9 (HA750302).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 9 (ET1612-77, red) at 1/50 dilution at +4℃ overnight, washed with PBS.
Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunocytochemistry analysis of MCF-7 cells labeling Cytokeratin 9 (HA750302).
Cells were fixed in 4% paraformaldehyde and permeabilized with 0.05% Triton X-100 in PBS for 10 minutes, and then blocked with 2% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody Cytokeratin 9 (ET1612-77, red) at 1/200 dilution for overnight at 4℃.
Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/800 dilution. DAPI was used to stain the cell nuclei (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"