NG2 Recombinant Rabbit Monoclonal Antibody [JM10-13] - BSA and Azide free
Catalog# HA750372
NG2 Recombinant Rabbit Monoclonal Antibody [JM10-13] - BSA and Azide free
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
NG2 Recombinant Rabbit Monoclonal Antibody [JM10-13] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human NG2 aa 2,271-2,314 / 2,322.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 251 kDa
Positive Control
A375 cell lysate, SK-MEL-28 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human malignant melanoma tissue, mouse brain tissue, rat brain tissue.
Conjugation
unconjugated
Clone Number
JM10-13
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
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IHC-P
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1:50-1:400
Target
Function
Chondroitin sulfate proteoglycan 4, also known as melanoma-associated chondroitin sulfate proteoglycan (MCSP) or neuron-glial antigen 2 (NG2), is a chondroitin sulfate proteoglycan that in humans is encoded by the CSPG4 gene. CSPG4 plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. It represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. CSPG4/NG2 is also a hallmark protein of oligodendrocyte progenitor cells (OPCs)[8] and OPC dysfunction has been implicated as a candidate pathophysiological mechanism of familial schizophrenia.
Background References
1. Raha-Chowdhury R et al. Expression and cellular localization of hepcidin mRNA and protein in normal rat brain. BMC Neurosci 16:24 (2015).
2. Milesi S et al. Redistribution of PDGFR cells and NG2DsRed pericytes at the cerebrovasculature after status epilepticus. Neurobiol Dis 71C:151-158 (2014).
Tissue Specificity
Detected only in malignant melanoma cells.
Post-translational Modification
O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.; Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
Subcellular Location
Cell membrane, Apical cell membrane, Cell projection, Cell surface.
Synonyms
4732461B14Rik antibody
AN2 antibody
AN2 proteoglycan antibody
Chondroitin sulfate proteoglycan 4 (melanoma-associated) antibody
Chondroitin sulfate proteoglycan 4 antibody
Chondroitin sulfate proteoglycan NG2 antibody
Cspg4 antibody
Cspg4 chondroitin sulfate proteoglycan 4 antibody
CSPG4_HUMAN antibody
HMW-MAA antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of NG2 on different lysates with Rabbit anti-NG2 antibody (HA750372) at 1/2,000 dilution.
Lane 1: A375 cell lysate (15 µg/Lane)
Lane 2: SK-MEL-28 cell lysate (15 µg/Lane)
Lane 3: MCF7 cell lysate (negative) (15 µg/Lane)
Lane 4: Mouse brain tissue lysate (20 µg/Lane)
Lane 5: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 251 kDa
Observed band size: 300 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750372) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue using anti-NG2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750372, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NG2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750372, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NG2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750372, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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