NG2 Recombinant Rabbit Monoclonal Antibody [JM10-13] (ET1703-16)
Catalog# ET1703-16
Rabbit Monoclonal to NG2
Tested for
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WB
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IHC-P
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of NG2 on different lysates with Rabbit anti-NG2 antibody (ET1703-16) at 1/2,000 dilution.
Lane 1: A375 cell lysate (15 µg/Lane)
Lane 2: SK-MEL-28 cell lysate (15 µg/Lane)
Lane 3: MCF7 cell lysate (negative) (15 µg/Lane)
Lane 4: Mouse brain tissue lysate (20 µg/Lane)
Lane 5: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 251 kDa
Observed band size: 300 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-16) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A375 cell lysate (15 µg/Lane)
Lane 2: SK-MEL-28 cell lysate (15 µg/Lane)
Lane 3: MCF7 cell lysate (negative) (15 µg/Lane)
Lane 4: Mouse brain tissue lysate (20 µg/Lane)
Lane 5: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 251 kDa
Observed band size: 300 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-16) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.