EDG1 Recombinant Rabbit Monoclonal Antibody [JM10-66] - BSA and Azide free
Catalog# HA750376
EDG1 Recombinant Rabbit Monoclonal Antibody [JM10-66] - BSA and Azide free
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WB
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IP
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Human
-
Mouse
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Rat
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unconjugated
Overview
Product Name
EDG1 Recombinant Rabbit Monoclonal Antibody [JM10-66] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human EDG1 aa 2-51 / 382.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IP
Target Molecular Weight
Predicted band size: 43 kDa
Positive Control
SH-SY5Y cell lysate, HepG2 cell lysate, bEnd.3 cell lysate, HepG2, HUVEC, SH-SY5Y, human breast carcinoma tissue, human liver tissue, mouse brain tissue, mouse heart tissue.
Conjugation
unconjugated
Clone Number
JM10-66
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000-5,000
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IP
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Use at an assay dependent concentration.
Target
Function
Sphingosine-1-phosphate receptor 1 (S1P receptor 1 or S1PR1), also known as endothelial differentiation gene 1 (EDG1) is a protein that in humans is encoded by the S1PR1 gene. S1PR1 is a G-protein-coupled receptor which binds the bioactive signaling molecule sphingosine 1-phosphate (S1P). S1PR1 belongs to a sphingosine-1-phosphate receptor subfamily comprising five members (S1PR1-5). S1PR1 was originally identified as an abundant transcript in endothelial cells and it has an important role in regulating endothelial cell cytoskeletal structure, migration, capillary-like network formation and vascular maturation. In addition, S1PR1 signaling is important in the regulation of lymphocyte maturation, migration and trafficking. Like the other members of the GPCR family, S1PR1 senses its ligand from outside the cell and activates intracellular signal pathways that at last lead to cellular responses. The signal is transduced through the association of the receptor with different G proteins, which recruits a series of systems for downstream amplification of the signal.
Background References
1. Alfonso J et al. Downregulation of Sphingosine 1-Phosphate Receptor 1 Promotes the Switch from Tangential to Radial Migration in the OB. J Neurosci 35:13659-72 (2015).
2. Bot M et al. Hematopoietic sphingosine 1-phosphate lyase deficiency decreases atherosclerotic lesion development in LDL-receptor deficient mice. PLoS One 8:e63360 (2013).
Sequence Similarity
Belongs to the G-protein coupled receptor 1 family.
Tissue Specificity
Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.
Post-translational Modification
S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue.
Subcellular Location
Cell membrane.
Synonyms
CD363 antibody
CHEDG 1 antibody
CHEDG1 antibody
D1S3362 antibody
ECGF 1 antibody
ECGF1 antibody
EDG 1 antibody
EDG1 antibody
endothelial differentiation G protein coupled receptor 1 antibody
Endothelial differentiation G-protein coupled receptor 1 antibody
ExpandImages
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Western blot analysis of EDG1 on different lysates with Rabbit anti-EDG1 antibody (HA750376) at 1/5,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: HepG2 cell lysate
Lane 3: bEnd.3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 1 minute 21 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750376) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of EDG1 on different lysates with Rabbit anti-EDG1 antibody (HA750376) at 1/5,000 dilution.
Lane 1: HepG2-si NT cell lysate
Lane 2: HepG2-si EDG1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750376) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of EDG1 on different lysates with Rabbit anti-EDG1 antibody (HA750376) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Rat brain tissue lysate
Lane 4: Rat spleen tissue lysate
Lysates/proteins at 20/40 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 60 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750376) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
EDG1 was immunoprecipitated in 0.2mg HepG2 cell lysate with HA750376 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750376 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate (input)
Lane 2: HA750376 IP in HepG2 cell lysate
Lane 3: Rabbit IgG instead of HA750376 in HepG2 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 4 mintues
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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