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EDG1 Recombinant Rabbit Monoclonal Antibody [JM10-66]

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Specification

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Catalog# ET1703-27

EDG1 Recombinant Rabbit Monoclonal Antibody [JM10-66]

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

EDG1 Recombinant Rabbit Monoclonal Antibody [JM10-66]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human EDG1 aa 2-51 / 382.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 43 kDa

Positive Control

SH-SY5Y cell lysate, HepG2 cell lysate, bEnd.3 cell lysate, HepG2, HUVEC, SH-SY5Y, human breast carcinoma tissue, human liver tissue, mouse brain tissue, mouse heart tissue.

Conjugation

unconjugated

Clone Number

JM10-66

RRID

AB_3070384

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-5,000

  • IP

  • Use at an assay dependent concentration.

Target

Function

Sphingosine-1-phosphate receptor 1 (S1P receptor 1 or S1PR1), also known as endothelial differentiation gene 1 (EDG1) is a protein that in humans is encoded by the S1PR1 gene. S1PR1 is a G-protein-coupled receptor which binds the bioactive signaling molecule sphingosine 1-phosphate (S1P). S1PR1 belongs to a sphingosine-1-phosphate receptor subfamily comprising five members (S1PR1-5). S1PR1 was originally identified as an abundant transcript in endothelial cells and it has an important role in regulating endothelial cell cytoskeletal structure, migration, capillary-like network formation and vascular maturation. In addition, S1PR1 signaling is important in the regulation of lymphocyte maturation, migration and trafficking. Like the other members of the GPCR family, S1PR1 senses its ligand from outside the cell and activates intracellular signal pathways that at last lead to cellular responses. The signal is transduced through the association of the receptor with different G proteins, which recruits a series of systems for downstream amplification of the signal.

Background References

1. Alfonso J et al. Downregulation of Sphingosine 1-Phosphate Receptor 1 Promotes the Switch from Tangential to Radial Migration in the OB. J Neurosci 35:13659-72 (2015).

2. Bot M et al. Hematopoietic sphingosine 1-phosphate lyase deficiency decreases atherosclerotic lesion development in LDL-receptor deficient mice. PLoS One 8:e63360 (2013).

Sequence Similarity

Belongs to the G-protein coupled receptor 1 family.

Tissue Specificity

Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.

Post-translational Modification

S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P21453 Human

SwissProt: O08530 Mouse

SwissProt: P48303 Rat

Synonyms

CD363 antibody

CHEDG 1 antibody

CHEDG1 antibody

D1S3362 antibody

ECGF 1 antibody

ECGF1 antibody

EDG 1 antibody

EDG1 antibody

endothelial differentiation G protein coupled receptor 1 antibody

Endothelial differentiation G-protein coupled receptor 1 antibody

Expand

Images

  • Western blot analysis of EDG1 on different lysates with Rabbit anti-EDG1 antibody (<a href="/products/ET1703-27" style="font-weight: bold;text-decoration: underline;">ET1703-27</a>) at 1/5,000 dilution.<br /><br />Lane 1: SH-SY5Y cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: bEnd.3 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 43 kDa<br />Observed band size: 43 kDa<br /><br />Exposure time: 1 minute 21 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-27" style="font-weight: bold;text-decoration: underline;">ET1703-27</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of EDG1 on different lysates with Rabbit anti-EDG1 antibody (ET1703-27) at 1/5,000 dilution.

    Lane 1: SH-SY5Y cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: bEnd.3 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

    Exposure time: 1 minute 21 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-27) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of EDG1 on different lysates with Rabbit anti-EDG1 antibody (<a href="/products/ET1703-27" style="font-weight: bold;text-decoration: underline;">ET1703-27</a>) at 1/5,000 dilution.<br /><br />Lane 1: HepG2-si NT cell lysate<br />Lane 2: HepG2-si EDG1 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 43 kDa<br />Observed band size: 43 kDa<br /><br />Exposure time: 2 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-27" style="font-weight: bold;text-decoration: underline;">ET1703-27</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of EDG1 on different lysates with Rabbit anti-EDG1 antibody (ET1703-27) at 1/5,000 dilution.

    Lane 1: HepG2-si NT cell lysate
    Lane 2: HepG2-si EDG1 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

    Exposure time: 2 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-27) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of EDG1 on different lysates with Rabbit anti-EDG1 antibody (<a href="/products/ET1703-27" style="font-weight: bold;text-decoration: underline;">ET1703-27</a>) at 1/2,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: PC-12 cell lysate<br />Lane 3: Rat brain tissue lysate<br />Lane 4: Rat spleen tissue lysate<br /><br />Lysates/proteins at 20/40 µg/Lane.<br /><br />Predicted band size: 43 kDa<br />Observed band size: 43 kDa<br /><br />Exposure time: 60 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-27" style="font-weight: bold;text-decoration: underline;">ET1703-27</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of EDG1 on different lysates with Rabbit anti-EDG1 antibody (ET1703-27) at 1/2,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: PC-12 cell lysate
    Lane 3: Rat brain tissue lysate
    Lane 4: Rat spleen tissue lysate

    Lysates/proteins at 20/40 µg/Lane.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

    Exposure time: 60 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-27) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • EDG1 was immunoprecipitated in 0.2mg HepG2 cell lysate with <a href="/products/ET1703-27" style="font-weight: bold;text-decoration: underline;">ET1703-27</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/ET1703-27" style="font-weight: bold;text-decoration: underline;">ET1703-27</a> at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HepG2 cell lysate (input)<br />Lane 2: <a href="/products/ET1703-27" style="font-weight: bold;text-decoration: underline;">ET1703-27</a> IP in HepG2 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/ET1703-27" style="font-weight: bold;text-decoration: underline;">ET1703-27</a> in HepG2 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 4 mintues

    EDG1 was immunoprecipitated in 0.2mg HepG2 cell lysate with ET1703-27 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1703-27 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HepG2 cell lysate (input)
    Lane 2: ET1703-27 IP in HepG2 cell lysate
    Lane 3: Rabbit IgG instead of ET1703-27 in HepG2 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 4 mintues

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

EDG1 Recombinant Rabbit Monoclonal Antibody [JM10-66] - BSA and Azide free

Application: WB,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated