RFP Recombinant Rabbit Monoclonal Antibody [JA10-52] - BSA and Azide free
Catalog# HA750408
RFP Recombinant Rabbit Monoclonal Antibody [JA10-52] - BSA and Azide free
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WB
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Species independent
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unconjugated
Overview
Product Name
RFP Recombinant Rabbit Monoclonal Antibody [JA10-52] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Entacmaea quadricolor eqFP611 RFP aa 1-216 / 231.
Species Reactivity
Species independent
Validated Applications
WB
Target Molecular Weight
Predicted band size: 26 kDa
Positive Control
RFP recombinant protein.
Conjugation
unconjugated
Clone Number
JA10-52
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
Target
Function
Red fluorescent protein cloned from Entacmaea quadricolor eqFP611 (Uniprot IDQ8ISF8)is an important tool in biotechnology and cell biology as a spectrally distinct companion or substitute for the green fluorescent protein (GFP; from Aequorea jellyfish).
Background References
1. Szymanska E et al. Impaired dynamin 2 function leads to increased AP-1 transcriptional activity through the JNK/c-Jun pathway. Cell Signal 28:160-71 (2016).
2. McWilliams TG et al. mito-QC illuminates mitophagy and mitochondrial architecture in vivo. J Cell Biol 214:333-45 (2016).
Sequence Similarity
Belongs to the GFP family.
Post-translational Modification
Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), oxidation of Tyr-(N+1) to didehydrotyrosine, and formation of a double bond to the alpha-amino nitrogen of residue Xaa-N. Maturation of the chromophore requires nothing other than molecular oxygen.
Synonyms
DsRed antibody
GFP like chromoprotein antibody
red fluorescent protein antibody
RFP antibody
Images
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Western blot analysis of RFP on RFP recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750408) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"