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RFP Recombinant Rabbit Monoclonal Antibody [JA10-52]

Usd: 385

Specification

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Catalog# ET1704-21

RFP Recombinant Rabbit Monoclonal Antibody [JA10-52]

Application

  • WB

Reactivity

  • Species independent

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

RFP Recombinant Rabbit Monoclonal Antibody [JA10-52]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Entacmaea quadricolor eqFP611 RFP aa 1-216 / 231.

Species Reactivity

Species independent

Validated Applications

WB

Molecular Weight

Predicted band size: 26 kDa

Positive Control

RFP recombinant protein.

Conjugation

unconjugated

Clone Number

JA10-52

RRID

AB_3070472

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

Target

Function

Red fluorescent protein cloned from Entacmaea quadricolor eqFP611 (Uniprot IDQ8ISF8)is an important tool in biotechnology and cell biology as a spectrally distinct companion or substitute for the green fluorescent protein (GFP; from Aequorea jellyfish).

Background References

1. Szymanska E et al. Impaired dynamin 2 function leads to increased AP-1 transcriptional activity through the JNK/c-Jun pathway. Cell Signal 28:160-71 (2016).

2. McWilliams TG et al. mito-QC illuminates mitophagy and mitochondrial architecture in vivo. J Cell Biol 214:333-45 (2016).

Sequence Similarity

Belongs to the GFP family.

Post-translational Modification

Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), oxidation of Tyr-(N+1) to didehydrotyrosine, and formation of a double bond to the alpha-amino nitrogen of residue Xaa-N. Maturation of the chromophore requires nothing other than molecular oxygen.

UNIPROT

SwissProt: Q8ISF8 EntacmaeaQuadricolorEqFP611

Synonyms

DsRed antibody

GFP like chromoprotein antibody

red fluorescent protein antibody

RFP antibody

Images

  • Western blot analysis of RFP on RFP recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1704-21" style="font-weight: bold;text-decoration: underline;">ET1704-21</a>) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of RFP on RFP recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-21) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation