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ACE Recombinant Rabbit Monoclonal Antibody [JM59-32] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750429

ACE Recombinant Rabbit Monoclonal Antibody [JM59-32] - BSA and Azide free

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

ACE Recombinant Rabbit Monoclonal Antibody [JM59-32] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human ACE aa 1,257-1,306 / 1,306.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC

Molecular Weight

Predicted band size: 150 kDa

Positive Control

Human lung tissue lysate, mouse lung tissue lysate, mouse kidney tissue lysate, human kidney tissue, mouse testis tissue, mouse kidney tissue.

Conjugation

unconjugated

Clone Number

JM59-32

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:1,000

  • FC

  • 1:50-1:100

Target

Function

Angiotensin-converting enzyme (EC 3.4.15.1), or ACE, is a central component of the renin–angiotensin system (RAS), which controls blood pressure by regulating the volume of fluids in the body. It converts the hormone angiotensin I to the active vasoconstrictor angiotensin II. Therefore, ACE indirectly increases blood pressure by causing blood vessels to constrict. ACE inhibitors are widely used as pharmaceutical drugs for treatment of cardiovascular diseases. Other lesser known functions of ACE are degradation of bradykinin, substance P and amyloid beta-protein. ACE hydrolyzes peptides by the removal of a dipeptide from the C-terminus. Likewise it converts the inactive decapeptide angiotensin I to the octapeptide angiotensin II by removing the dipeptide His-Leu. Angiotensin II is a potent vasoconstrictor in a substrate concentration-dependent manner. Angiotensin II binds to the type 1 angiotensin II receptor (AT1), which sets off a number of actions that result in vasoconstriction and therefore increased blood pressure. ACE is also part of the kinin-kallikrein system where it degrades bradykinin, a potent vasodilator, and other vasoactive peptides. Kininase II is the same as angiotensin-converting enzyme. Thus, the same enzyme (ACE) that generates a vasoconstrictor (ANG II) also disposes of vasodilators (bradykinin).

Background References

1. Álvarez-Zaballos S et al. Angiotensin-Converting Enzyme and Heart Failure. Front Biosci (Landmark Ed). 2023 Jul

2. Khurana V et al. Angiotensin converting enzyme (ACE). Clin Chim Acta. 2022 Jan

Sequence Similarity

Belongs to the peptidase M2 family.

Tissue Specificity

Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.

Post-translational Modification

Phosphorylated by CK2 on Ser-1299; which allows membrane retention.

Subcellular Location

Cell membrane, Cytoplasm; Secreted.

UNIPROT

SwissProt: P12821 Human

SwissProt: P09470 Mouse

Synonyms

ACE 1 antibody

ACE antibody

ACE T antibody

ACE_HUMAN antibody

ACE1 antibody

Angiotensin converting enzyme somatic isoform antibody

Angiotensin converting enzyme testis specific isoform antibody

Angiotensin I converting enzyme 1 antibody

Angiotensin I converting enzyme antibody

Angiotensin I converting enzyme peptidyl dipeptidase A 1 antibody

Expand

Images

  • Western blot analysis of ACE on different lysates with Rabbit anti-ACE antibody (<a href="/products/HA750429" style="font-weight: bold;text-decoration: underline;">HA750429</a>) at 1/5,000 dilution.<br /><br />Lane 1: Human lung tissue lysate<br />Lane 2: Mouse lung tissue lysate<br />Lane 3: Mouse kidney tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 150 kDa<br />Observed band size: 150-170 kDa<br /><br />Exposure time: 2 minutes 50 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750429" style="font-weight: bold;text-decoration: underline;">HA750429</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ACE on different lysates with Rabbit anti-ACE antibody (HA750429) at 1/5,000 dilution.

    Lane 1: Human lung tissue lysate
    Lane 2: Mouse lung tissue lysate
    Lane 3: Mouse kidney tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 150 kDa
    Observed band size: 150-170 kDa

    Exposure time: 2 minutes 50 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750429) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ACE antibody (<a href="/products/HA750429" style="font-weight: bold;text-decoration: underline;">HA750429</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750429" style="font-weight: bold;text-decoration: underline;">HA750429</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ACE antibody (HA750429) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750429) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ACE antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750429" style="font-weight: bold;text-decoration: underline;">HA750429</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ACE antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750429, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ACE antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750429" style="font-weight: bold;text-decoration: underline;">HA750429</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ACE antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750429, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of ACE on rat kidney tissue lysates with Rabbit anti-ACE antibody (<a href="/products/HA750429" style="font-weight: bold;text-decoration: underline;">HA750429</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 150 kDa<br />Observed band size: 170 kDa<br /><br />Exposure time: 1 minutes 150 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750429" style="font-weight: bold;text-decoration: underline;">HA750429</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ACE on rat kidney tissue lysates with Rabbit anti-ACE antibody (HA750429) at 1/1,000 dilution.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 150 kDa
    Observed band size: 170 kDa

    Exposure time: 1 minutes 150 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750429) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Products with the same target and pathway

metafield image

ACE Recombinant Rabbit Monoclonal Antibody [JM59-32]

Application: WB,IHC-P,FC,mIHC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated