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ACSL4 / FACL4 Recombinant Rabbit Monoclonal Antibody [JE56-33] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750469

ACSL4 / FACL4 Recombinant Rabbit Monoclonal Antibody [JE56-33] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

ACSL4 / FACL4 Recombinant Rabbit Monoclonal Antibody [JE56-33] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human FACL4 aa 561-711 (Cytoplasmic).

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 79 kDa

Positive Control

PC-12 cell lysate, C6 cell lysate, L6 cell lysate, Rat brain tissue lysate, Rat lung tissue lysate, Rat thymus tissue lysate, A549 cell lysate, Mouse brain tissue lysate, mouse brain tissue, mouse lung tissue, rat brain tissue.

Conjugation

unconjugated

Clone Number

JE56-33

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:1,000

Target

Function

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants.

Background References

1. Cheng J. et. al. ACSL4 suppresses glioma cells proliferation via activating ferroptosis. Oncol Rep. 2020 Jan

2. Kuwata H. et. al. Role of acyl-CoA synthetase ACSL4 in arachidonic acid metabolism. Prostaglandins Other Lipid Mediat. 2019 Oct

Subcellular Location

Peroxisome membrane, Mitochondrion outer membrane, Microsome membrane, Endoplasmic reticulum membrane, Cell membrane.

UNIPROT

SwissProt: O60488 Human

SwissProt: Q9QUJ7 Mouse

SwissProt: O35547 Rat

Synonyms

ACS 4 antibody

ACS4 antibody

ACSL 4 antibody

Acsl4 antibody

ACSL4_HUMAN antibody

acyl CoA synthetase 4 antibody

Acyl CoA synthetase long chain family member 4 antibody

FACL 4 antibody

FACL4 antibody

Fatty acid Coenzyme A ligase antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of ACSL4 / FACL4 on different lysates with Rabbit anti-ACSL4 / FACL4 antibody (<a href="/products/HA750469" style="font-weight: bold;text-decoration: underline;">HA750469</a>) at 1/2,000 dilution.<br /><br />Lane 1: Huh7 (Human liver cancer cell) cell lysate<br />Lane 2: Hep G2 (Human liver cancer cell) cell lysate<br />Lane 3: MCF7 (Human breast cancer cell) cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 10 seconds; ECL: K1801<br /><br />Negative expression of ACSL4 / FACL4 protein in MCF7 is consistent with the predicted expression pattern.<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA750469" style="font-weight: bold;text-decoration: underline;">HA750469</a>, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 79 kDa<br />Observed band size: 75 kDa

    ☑ Relative expression (RE)

    Western blot analysis of ACSL4 / FACL4 on different lysates with Rabbit anti-ACSL4 / FACL4 antibody (HA750469) at 1/2,000 dilution.

    Lane 1: Huh7 (Human liver cancer cell) cell lysate
    Lane 2: Hep G2 (Human liver cancer cell) cell lysate
    Lane 3: MCF7 (Human breast cancer cell) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Negative expression of ACSL4 / FACL4 protein in MCF7 is consistent with the predicted expression pattern.

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA750469, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 79 kDa
    Observed band size: 75 kDa

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of ACSL4 / FACL4 on different lysates with Rabbit anti-ACSL4 / FACL4 antibody (<a href="/products/HA750469" style="font-weight: bold;text-decoration: underline;">HA750469</a>) at 1/5,000 dilution.<br /><br />Lane 1: A549-si NT cell lysate<br />Lane 2: A549-si ACSL4 / FACL4 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 79 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750469" style="font-weight: bold;text-decoration: underline;">HA750469</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of ACSL4 / FACL4 on different lysates with Rabbit anti-ACSL4 / FACL4 antibody (HA750469) at 1/5,000 dilution.

    Lane 1: A549-si NT cell lysate
    Lane 2: A549-si ACSL4 / FACL4 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 79 kDa
    Observed band size: 75 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750469) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of ACSL4 / FACL4 on different lysates with Rabbit anti-ACSL4 / FACL4 antibody (<a href="/products/HA750469" style="font-weight: bold;text-decoration: underline;">HA750469</a>) at 1/1,000 dilution.<br /><br />Lane 1: PC-12 cell lysate<br />Lane 2: C6 cell lysate<br />Lane 3: L6 cell lysate<br />Lane 4: Rat brain tissue lysate<br />Lane 5: Rat lung tissue lysate<br />Lane 6: Rat thymus tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 79 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 42 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750469" style="font-weight: bold;text-decoration: underline;">HA750469</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ACSL4 / FACL4 on different lysates with Rabbit anti-ACSL4 / FACL4 antibody (HA750469) at 1/1,000 dilution.

    Lane 1: PC-12 cell lysate
    Lane 2: C6 cell lysate
    Lane 3: L6 cell lysate
    Lane 4: Rat brain tissue lysate
    Lane 5: Rat lung tissue lysate
    Lane 6: Rat thymus tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 79 kDa
    Observed band size: 75 kDa

    Exposure time: 42 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750469) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of ACSL4 / FACL4 on different lysates with Rabbit anti-ACSL4 / FACL4 antibody (<a href="/products/HA750469" style="font-weight: bold;text-decoration: underline;">HA750469</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549 cell lysate (20 µg/Lane)<br />Lane 2: Mouse brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 79 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750469" style="font-weight: bold;text-decoration: underline;">HA750469</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ACSL4 / FACL4 on different lysates with Rabbit anti-ACSL4 / FACL4 antibody (HA750469) at 1/1,000 dilution.

    Lane 1: A549 cell lysate (20 µg/Lane)
    Lane 2: Mouse brain tissue lysate (40 µg/Lane)

    Predicted band size: 79 kDa
    Observed band size: 75 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750469) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ACSL4 / FACL4 antibody (<a href="/products/HA750469" style="font-weight: bold;text-decoration: underline;">HA750469</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750469" style="font-weight: bold;text-decoration: underline;">HA750469</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ACSL4 / FACL4 antibody (HA750469) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750469) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-ACSL4 / FACL4 antibody (<a href="/products/HA750469" style="font-weight: bold;text-decoration: underline;">HA750469</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750469" style="font-weight: bold;text-decoration: underline;">HA750469</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-ACSL4 / FACL4 antibody (HA750469) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750469) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ACSL4 / FACL4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750469" style="font-weight: bold;text-decoration: underline;">HA750469</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ACSL4 / FACL4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750469, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

ACSL4 / FACL4 Recombinant Rabbit Monoclonal Antibody [JE56-33]

Application: WB,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated