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Villin1 Recombinant Rabbit Monoclonal Antibody [C4] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750476

Villin1 Recombinant Rabbit Monoclonal Antibody [C4] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Villin1 Recombinant Rabbit Monoclonal Antibody [C4] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Villin1 aa 600-827.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 93 kDa

Positive Control

HT-29 (Human colorectal adenocarcinoma cell) cell lysate, Caco-2 (Human colorectal adenocarcinoma cell) cell lysate, Hep G2 (Human hepatocellular carcinoma cell) cell lysate, Mouse small intestine tissue lysate, Mouse kidney tissue lysate, Rat small intestine tissue lysate, human kidney tissue, human small intestine tissue, mouse small intestine tissue, rat small intestine tissue.

Conjugation

unconjugated

Clone Number

C4

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:10,000

  • IHC-P

  • 1:3,500-1:20,000

Target

Function

This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.

Background References

1. Nasser MET. et. al. Value of Plasmatic Villin-1 in the Prediction of Early Graft Dysfunction in Kidney Transplant Recipients: Diagnostic Accuracy Test. Exp Clin Transplant. 2021 Feb

2. Roy S. et. al. Villin-1 and Gelsolin Regulate Changes in Actin Dynamics That Affect Cell Survival Signaling Pathways and Intestinal Inflammation. Gastroenterology. 2018 Apr

Subcellular Location

Cytoskeleton, lamellipodium, ruffle, microvillus, filopodium tip, filopodium.

UNIPROT

SwissProt: P09327 Human

Synonyms

D2S1471 antibody

OTTHUMP00000164145 antibody

VIL antibody

VIL1 antibody

VILI_HUMAN antibody

Villin 1 antibody

Villin-1 antibody

Villin1 antibody

Images

  • Western blot analysis of Villin1 on different lysates with Rabbit anti-Villin1 antibody (<a href="/products/HA750476" style="font-weight: bold;text-decoration: underline;">HA750476</a>) at 1/10,000 dilution.<br /><br />Lane 1: HT-29 (Human colorectal adenocarcinoma cell) cell lysate<br />Lane 2: Caco-2 (Human colorectal adenocarcinoma cell) cell lysate<br />Lane 3: Hep G2 (Human hepatocellular carcinoma cell) cell lysate<br />Lane 4: Mouse small intestine tissue lysate<br />Lane 5: Mouse kidney tissue lysate<br />Lane 6: Rat small intestine tissue lysate<br /><br />Cell Lysates/proteins at 15 µg/Lane, Tissue Lysates/proteins at 30 µg/Lane.<br />Exposure time: 17 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA750476" style="font-weight: bold;text-decoration: underline;">HA750476</a>, 1/10,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 93 kDa<br />Observed band size: 93 kDa

    Western blot analysis of Villin1 on different lysates with Rabbit anti-Villin1 antibody (HA750476) at 1/10,000 dilution.

    Lane 1: HT-29 (Human colorectal adenocarcinoma cell) cell lysate
    Lane 2: Caco-2 (Human colorectal adenocarcinoma cell) cell lysate
    Lane 3: Hep G2 (Human hepatocellular carcinoma cell) cell lysate
    Lane 4: Mouse small intestine tissue lysate
    Lane 5: Mouse kidney tissue lysate
    Lane 6: Rat small intestine tissue lysate

    Cell Lysates/proteins at 15 µg/Lane, Tissue Lysates/proteins at 30 µg/Lane.
    Exposure time: 17 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA750476, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 93 kDa
    Observed band size: 93 kDa

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Villin1 antibody (<a href="/products/HA750476" style="font-weight: bold;text-decoration: underline;">HA750476</a>) at 1/3,500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750476" style="font-weight: bold;text-decoration: underline;">HA750476</a>) at 1/3,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Villin1 antibody (HA750476) at 1/3,500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750476) at 1/3,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Villin1 antibody (<a href="/products/HA750476" style="font-weight: bold;text-decoration: underline;">HA750476</a>) at 1/3,500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750476" style="font-weight: bold;text-decoration: underline;">HA750476</a>) at 1/3,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Villin1 antibody (HA750476) at 1/3,500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750476) at 1/3,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Mouse<br />Tissue: Small intestine<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA750476" style="font-weight: bold;text-decoration: underline;">HA750476</a>, 1/20,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Small intestine
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA750476, 1/20,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Rat<br />Tissue: Small intestine<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA750476" style="font-weight: bold;text-decoration: underline;">HA750476</a>, 1/20,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Small intestine
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA750476, 1/20,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"