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hSET1/SET1 Recombinant Rabbit Monoclonal Antibody [PH00-01] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750554

hSET1/SET1 Recombinant Rabbit Monoclonal Antibody [PH00-01] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

With BSA and Azide
Predicted reactivity

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

hSET1/SET1 Recombinant Rabbit Monoclonal Antibody [PH00-01] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within C terminal human SET1A.

Species Reactivity

Human (Predicted: Mouse, Rat)

Predicted species support after-sales service

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 186 kDa

Positive Control

HeLa cell lysate,293T cell lysate, Jurkat cell lysate, HepG2 cell lysate,human lung carcinoma tissue, human testis tissue, mouse hippocampus tissue.

Conjugation

unconjugated

Clone Number

PH00-01

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:400

Target

Function

Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism. Part of chromatin remodeling machinery, forms H3K4me1, H3K4me2 and H3K4me3 methylation marks at active chromatin sites where transcription and DNA repair take place. Responsible for H3K4me3 enriched promoters and transcriptional programming of inner mass stem cells and neuron progenitors during embryogenesis. Required for H3K4me1 mark at stalled replication forks. Mediates FANCD2-dependent nucleosome remodeling and RAD51 nucleofilaments stabilization at reversed forks, protecting them from nucleolytic degradation. Does not methylate 'Lys-4' of histone H3 if the neighboring 'Lys-9' residue is already methylated.

Background References

1. Kummeling J., et al. Characterization of SETD1A haploinsufficiency in humans and Drosophila defines a novel neurodevelopmental syndrome. Mol. Psychiatry 26:2013-2024 (2021)

2. Wysocka J., Myers M.P., Laherty C.D., Eisenman R.N., Herr W. Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell- proliferation factor HCF-1. Genes Dev. 17:896-911 (2003)

Subcellular Location

Chromosome, Nucleus

UNIPROT

SwissProt: O15047 Human

SwissProt: E9PYH6 Mouse

SwissProt: A0A8I6AS32 Rat

Synonyms

Histone-lysine N-methyltransferase SETD1A antibody

hSET1A antibody

KMT2F antibody

Lysine N methyltransferase 2F antibody

Lysine N-methyltransferase 2F antibody

SET domain containing 1A antibody

SET domain containing protein 1A antibody

SET domain-containing protein 1A antibody

SET1 antibody

Set1 Ash2 histone methyltransferase complex subunit SET1 antibody

Expand

Images

  • Western blot analysis of hSET1/SET1 on different lysates with Rabbit anti-hSET1/SET1 antibody (<a href="/products/HA750554" style="font-weight: bold;text-decoration: underline;">HA750554</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate<br />Lane 2: 293T (Human embryonic kidney cell) cell lysate<br />Lane 3: Jurkat (Human T-lymphoblastic cells) cell lysate<br />Lane 4: HepG2 (Human liver cancer cells) cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 90 seconds<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA750554" style="font-weight: bold;text-decoration: underline;">HA750554</a>, 1/1,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/200,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 186 kDa<br />Observed band size: 250~300 kDa

    Western blot analysis of hSET1/SET1 on different lysates with Rabbit anti-hSET1/SET1 antibody (HA750554) at 1/1,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
    Lane 2: 293T (Human embryonic kidney cell) cell lysate
    Lane 3: Jurkat (Human T-lymphoblastic cells) cell lysate
    Lane 4: HepG2 (Human liver cancer cells) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 90 seconds

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA750554, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/200,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 186 kDa
    Observed band size: 250~300 kDa

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-hSET1/SET1 antibody (<a href="/products/HA750554" style="font-weight: bold;text-decoration: underline;">HA750554</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750554" style="font-weight: bold;text-decoration: underline;">HA750554</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-hSET1/SET1 antibody (HA750554) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750554) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-hSET1/SET1 antibody (<a href="/products/HA750554" style="font-weight: bold;text-decoration: underline;">HA750554</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750554" style="font-weight: bold;text-decoration: underline;">HA750554</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-hSET1/SET1 antibody (HA750554) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750554) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-hSET1/SET1 antibody (<a href="/products/HA750554" style="font-weight: bold;text-decoration: underline;">HA750554</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750554" style="font-weight: bold;text-decoration: underline;">HA750554</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-hSET1/SET1 antibody (HA750554) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750554) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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