hSET1/SET1 Recombinant Rabbit Monoclonal Antibody [PH00-01] - BSA and Azide free
Catalog# HA750554
hSET1/SET1 Recombinant Rabbit Monoclonal Antibody [PH00-01] - BSA and Azide free
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
hSET1/SET1 Recombinant Rabbit Monoclonal Antibody [PH00-01] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within C terminal human SET1A.
Species Reactivity
Human (Predicted: Mouse, Rat)
Predicted species support after-sales service
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 186 kDa
Positive Control
HeLa cell lysate,293T cell lysate, Jurkat cell lysate, HepG2 cell lysate,human lung carcinoma tissue, human testis tissue, mouse hippocampus tissue.
Conjugation
unconjugated
Clone Number
PH00-01
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism. Part of chromatin remodeling machinery, forms H3K4me1, H3K4me2 and H3K4me3 methylation marks at active chromatin sites where transcription and DNA repair take place. Responsible for H3K4me3 enriched promoters and transcriptional programming of inner mass stem cells and neuron progenitors during embryogenesis. Required for H3K4me1 mark at stalled replication forks. Mediates FANCD2-dependent nucleosome remodeling and RAD51 nucleofilaments stabilization at reversed forks, protecting them from nucleolytic degradation. Does not methylate 'Lys-4' of histone H3 if the neighboring 'Lys-9' residue is already methylated.
Background References
1. Kummeling J., et al. Characterization of SETD1A haploinsufficiency in humans and Drosophila defines a novel neurodevelopmental syndrome. Mol. Psychiatry 26:2013-2024 (2021)
2. Wysocka J., Myers M.P., Laherty C.D., Eisenman R.N., Herr W. Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell- proliferation factor HCF-1. Genes Dev. 17:896-911 (2003)
Subcellular Location
Chromosome, Nucleus
Synonyms
Histone-lysine N-methyltransferase SETD1A antibody
hSET1A antibody
KMT2F antibody
Lysine N methyltransferase 2F antibody
Lysine N-methyltransferase 2F antibody
SET domain containing 1A antibody
SET domain containing protein 1A antibody
SET domain-containing protein 1A antibody
SET1 antibody
Set1 Ash2 histone methyltransferase complex subunit SET1 antibody
ExpandImages
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This data was developed using HA721224, the same antibody clone in a different buffer formulation.
Western blot analysis of hSET1/SET1 on different lysates with Rabbit anti-hSET1/SET1 antibody (HA721224) at 1/1,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: 293T (Human embryonic kidney cell) cell lysate
Lane 3: Jurkat (Human T-lymphoblastic cells) cell lysate
Lane 4: HepG2 (Human liver cancer cells) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 90 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA721224, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/200,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 186 kDa
Observed band size: 250~300 kDa -
This data was developed using HA721224, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-hSET1/SET1 antibody (HA721224) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721224) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721224, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-hSET1/SET1 antibody (HA721224) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721224) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721224, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-hSET1/SET1 antibody (HA721224) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721224) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"