alpha Tubulin (acetyl K40) Recombinant Rabbit Monoclonal Antibody [PSH0-13] - BSA and Azide free
Catalog# HA750583
alpha Tubulin (acetyl K40) Recombinant Rabbit Monoclonal Antibody [PSH0-13] - BSA and Azide free
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WB
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IF-Cell
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Human
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Mouse
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Rat
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HA721271
含抗保成分
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unconjugated
Overview
Product Name
alpha Tubulin (acetyl K40) Recombinant Rabbit Monoclonal Antibody [PSH0-13] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human alpha Tubulin aa 30-45 / 451 (acetyl K40).
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell
Molecular Weight
Predicted band size: 50 kDa
Positive Control
HeLa cell lysate, HeLa treated with 500ng/mL TSA for 4 hours cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 400nM TSA for 18 hours cell lysate, C6 cell lysate, C6 treated with 1μM TSA for 18 hours cell lysate.
Conjugation
unconjugated
Clone Number
PSH0-13
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:250-1:3,500
Target
Function
Tubulin alpha-1B chain is a protein that in humans is encoded by the TUBA1B gene. TUBA1B has been shown to interact with PIK3R1. Antibodies against tubulin alpha 1b can be used as markers for microtubules and spindles. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Background References
1. Xu QQ et al. The Expression and Potential Role of Tubulin Alpha 1b in Wilms\' Tumor. Biomed Res Int. 2020 Aug
2. Hu X et al. Tubulin Alpha 1b Is Associated with the Immune Cell Infiltration and the Response of HCC Patients to Immunotherapy. Diagnostics (Basel). 2022 Mar
Subcellular Location
Cytoplasm, cytoskeleton.
Synonyms
Alpha tubulin ubiquitous antibody
Alpha-tubulin ubiquitous antibody
K alpha 1 antibody
TBA1B_HUMAN antibody
TUBA1B antibody
Tubulin alpha 1B antibody
Tubulin alpha 1B chain antibody
Tubulin alpha ubiquitous antibody
Tubulin alpha ubiquitous chain antibody
Tubulin alpha-1B chain antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of alpha Tubulin (acetyl K40) on different lysates with Rabbit anti-alpha Tubulin (acetyl K40) antibody (HA750583) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 500ng/mL TSA for 4 hours cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 400nM TSA for 18 hours cell lysate
Lane 5: C6 cell lysate
Lane 6: C6 treated with 1μM TSA for 18 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 55 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750583) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Immunocytochemistry analysis of HeLa cells treated with or without 500ng/mL TSA for 4 hours labeling alpha Tubulin (acetyl K40) with Rabbit anti-alpha Tubulin (acetyl K40) antibody (HA750583) at 1/250 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin (acetyl K40) antibody (HA750583) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
☑ Cell treatment (CT)
Immunocytochemistry analysis of NIH/3T3 cells treated with or without 400nM TSA for 18 hours labeling alpha Tubulin (acetyl K40) with Rabbit anti-alpha Tubulin (acetyl K40) antibody (HA750583) at 1/3,500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin (acetyl K40) antibody (HA750583) at 1/3,500 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
☑ Cell treatment (CT)
Immunocytochemistry analysis of C6 cells treated with or without 1μM TSA for 18 hours labeling alpha Tubulin (acetyl K40) with Rabbit anti-alpha Tubulin (acetyl K40) antibody (HA750583) at 1/3,500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin (acetyl K40) antibody (HA750583) at 1/3,500 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"