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alpha Tubulin (acetyl K40) Recombinant Rabbit Monoclonal Antibody [PSH0-13]

Usd: 385

Specification

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Catalog# HA721271

alpha Tubulin (acetyl K40) Recombinant Rabbit Monoclonal Antibody [PSH0-13]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

alpha Tubulin (acetyl K40) Recombinant Rabbit Monoclonal Antibody [PSH0-13]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human alpha Tubulin aa 30-45 / 451 (acetyl K40).

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 50 kDa

Positive Control

HeLa cell lysate, HeLa treated with 500ng/mL TSA for 4 hours cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 400nM TSA for 18 hours cell lysate, C6 cell lysate, C6 treated with 1μM TSA for 18 hours cell lysate.

Conjugation

unconjugated

Clone Number

PSH0-13

RRID

AB_3072388

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:250-1:3,500

Target

Function

Tubulin alpha-1B chain is a protein that in humans is encoded by the TUBA1B gene. TUBA1B has been shown to interact with PIK3R1. Antibodies against tubulin alpha 1b can be used as markers for microtubules and spindles. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Background References

1. Xu QQ et al. The Expression and Potential Role of Tubulin Alpha 1b in Wilms\' Tumor. Biomed Res Int. 2020 Aug

2. Hu X et al. Tubulin Alpha 1b Is Associated with the Immune Cell Infiltration and the Response of HCC Patients to Immunotherapy. Diagnostics (Basel). 2022 Mar

Subcellular Location

Cytoplasm, cytoskeleton.

UNIPROT

SwissProt: P68363 Human

SwissProt: P05213 Mouse

SwissProt: Q6P9V9 Rat

Synonyms

Alpha tubulin ubiquitous antibody

Alpha-tubulin ubiquitous antibody

K alpha 1 antibody

TBA1B_HUMAN antibody

TUBA1B antibody

Tubulin alpha 1B antibody

Tubulin alpha 1B chain antibody

Tubulin alpha ubiquitous antibody

Tubulin alpha ubiquitous chain antibody

Tubulin alpha-1B chain antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of alpha Tubulin (acetyl K40) on different lysates with Rabbit anti-alpha Tubulin (acetyl K40) antibody (<a href="/products/HA721271" style="font-weight: bold;text-decoration: underline;">HA721271</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HeLa treated with 500ng/mL TSA for 4 hours cell lysate<br />Lane 3: NIH/3T3 cell lysate<br />Lane 4: NIH/3T3 treated with 400nM TSA for 18 hours cell lysate<br />Lane 5: C6 cell lysate<br />Lane 6: C6 treated with 1μM TSA for 18 hours cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 50 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 4 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721271" style="font-weight: bold;text-decoration: underline;">HA721271</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of alpha Tubulin (acetyl K40) on different lysates with Rabbit anti-alpha Tubulin (acetyl K40) antibody (HA721271) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HeLa treated with 500ng/mL TSA for 4 hours cell lysate
    Lane 3: NIH/3T3 cell lysate
    Lane 4: NIH/3T3 treated with 400nM TSA for 18 hours cell lysate
    Lane 5: C6 cell lysate
    Lane 6: C6 treated with 1μM TSA for 18 hours cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 50 kDa
    Observed band size: 55 kDa

    Exposure time: 4 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721271) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Immunocytochemistry analysis of HeLa cells treated with or without 500ng/mL TSA for 4 hours labeling alpha Tubulin (acetyl K40) with Rabbit anti-alpha Tubulin (acetyl K40) antibody (<a href="/products/HA721271" style="font-weight: bold;text-decoration: underline;">HA721271</a>) at 1/250 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin (acetyl K40) antibody (<a href="/products/HA721271" style="font-weight: bold;text-decoration: underline;">HA721271</a>) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    ☑ Cell treatment (CT)

    Immunocytochemistry analysis of HeLa cells treated with or without 500ng/mL TSA for 4 hours labeling alpha Tubulin (acetyl K40) with Rabbit anti-alpha Tubulin (acetyl K40) antibody (HA721271) at 1/250 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin (acetyl K40) antibody (HA721271) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Immunocytochemistry analysis of NIH/3T3 cells treated with or without 400nM TSA for 18 hours labeling alpha Tubulin (acetyl K40) with Rabbit anti-alpha Tubulin (acetyl K40) antibody (<a href="/products/HA721271" style="font-weight: bold;text-decoration: underline;">HA721271</a>) at 1/3,500 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin (acetyl K40) antibody (<a href="/products/HA721271" style="font-weight: bold;text-decoration: underline;">HA721271</a>) at 1/3,500 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    ☑ Cell treatment (CT)

    Immunocytochemistry analysis of NIH/3T3 cells treated with or without 400nM TSA for 18 hours labeling alpha Tubulin (acetyl K40) with Rabbit anti-alpha Tubulin (acetyl K40) antibody (HA721271) at 1/3,500 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin (acetyl K40) antibody (HA721271) at 1/3,500 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Immunocytochemistry analysis of C6 cells treated with or without 1μM TSA for 18 hours labeling alpha Tubulin (acetyl K40) with Rabbit anti-alpha Tubulin (acetyl K40) antibody (<a href="/products/HA721271" style="font-weight: bold;text-decoration: underline;">HA721271</a>) at 1/3,500 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin (acetyl K40) antibody (<a href="/products/HA721271" style="font-weight: bold;text-decoration: underline;">HA721271</a>) at 1/3,500 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    ☑ Cell treatment (CT)

    Immunocytochemistry analysis of C6 cells treated with or without 1μM TSA for 18 hours labeling alpha Tubulin (acetyl K40) with Rabbit anti-alpha Tubulin (acetyl K40) antibody (HA721271) at 1/3,500 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin (acetyl K40) antibody (HA721271) at 1/3,500 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation