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CEACAM1 Recombinant Rabbit Monoclonal Antibody [PSH0-78] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750658

CEACAM1 Recombinant Rabbit Monoclonal Antibody [PSH0-78] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

CEACAM1 Recombinant Rabbit Monoclonal Antibody [PSH0-78] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human CEACAM1 aa 35-428 / 526.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 58 kDa

Positive Control

SW480 cell lysate, A549 cell lysate, human kidney tissue, human liver tissue.

Conjugation

unconjugated

Clone Number

PSH0-78

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

Carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) (CEACAM1) also known as CD66a (Cluster of Differentiation 66a), is a human glycoprotein, and a member of the carcinoembryonic antigen (CEA) gene family. This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell–cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of only two has been determined. In melanocytic cells CEACAM1 gene expression may be regulated by MITF.

Background References

1. Park DJ et al. EpCAM-high liver cancer stem cells resist natural killer cell-mediated cytotoxicity by upregulating CEACAM1. J Immunother Cancer. 2020 Mar

2. To SKY et al. A Selective β-Catenin-Metadherin/CEACAM1-CCL3 Axis Mediates Metastatic Heterogeneity upon Tumor-Macrophage Interaction. Adv Sci (Weinh). 2022 May

Subcellular Location

Cell membrane, Lateral cell membrane, Apical cell membrane, Basal cell membrane, Cell junction, adherens junction.

UNIPROT

SwissProt: P13688 Human

Synonyms

Antigen CD66 antibody

BGP 1 antibody

BGP antibody

BGP-1 antibody

BGPI antibody

Biliary glycoprotein 1 antibody

Biliary glycoprotein adhesion molecule antibody

Biliary glycoprotein antibody

Carcinoembryonic antigen related cell adhesion molecule 1 antibody

carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) antibody

Expand

Images

  • Western blot analysis of CEACAM1 on different lysates with Rabbit anti-CEACAM1 antibody (<a href="/products/HA750658" style="font-weight: bold;text-decoration: underline;">HA750658</a>) at 1/1,000 dilution and Mouse anti-6X His-tag antibody (HA600030) at 1/1,000 dilution.<br /><br />Lane 1: His-tagged human CEACAM-1 recombinant protein aa (aa35-428) 20ng<br />Lane 2: His-tagged human CEACAM-5 recombinant protein aa (aa35-422) 20ng<br />Lane 3: His-tagged human CEACAM-6 recombinant protein aa (aa35-326) 20ng<br />Lane 4: His-tagged human CEACAM-8 recombinant protein aa (aa35-335) 20ng<br /><br />Exposure time: 1 minute; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750658" style="font-weight: bold;text-decoration: underline;">HA750658</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CEACAM1 on different lysates with Rabbit anti-CEACAM1 antibody (HA750658) at 1/1,000 dilution and Mouse anti-6X His-tag antibody (HA600030) at 1/1,000 dilution.

    Lane 1: His-tagged human CEACAM-1 recombinant protein aa (aa35-428) 20ng
    Lane 2: His-tagged human CEACAM-5 recombinant protein aa (aa35-422) 20ng
    Lane 3: His-tagged human CEACAM-6 recombinant protein aa (aa35-326) 20ng
    Lane 4: His-tagged human CEACAM-8 recombinant protein aa (aa35-335) 20ng

    Exposure time: 1 minute; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750658) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CEACAM1 on different lysates with Rabbit anti-CEACAM1 antibody (<a href="/products/HA750658" style="font-weight: bold;text-decoration: underline;">HA750658</a>) at 1/1,000 dilution.<br /><br />Lane 1: SW480 cell lysate (20 µg/Lane)<br />Lane 2: SW480 cell lysate (no heat) (20 µg/Lane)<br />Lane 3: A549 cell lysate (20 µg/Lane)<br />Lane 4: HeLa cell lysate (no heat) (negative) (20 µg/Lane)<br />Lane 5: 293T cell lysate (negative) (20 µg/Lane)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Predicted band size: 58 kDa<br />Observed band size: 58-150 kDa<br /><br />Exposure time: 2 minutes;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750658" style="font-weight: bold;text-decoration: underline;">HA750658</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/100,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of CEACAM1 on different lysates with Rabbit anti-CEACAM1 antibody (HA750658) at 1/1,000 dilution.

    Lane 1: SW480 cell lysate (20 µg/Lane)
    Lane 2: SW480 cell lysate (no heat) (20 µg/Lane)
    Lane 3: A549 cell lysate (20 µg/Lane)
    Lane 4: HeLa cell lysate (no heat) (negative) (20 µg/Lane)
    Lane 5: 293T cell lysate (negative) (20 µg/Lane)

    Notice: no heat means the lysate is not boiled.

    Predicted band size: 58 kDa
    Observed band size: 58-150 kDa

    Exposure time: 2 minutes;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750658) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CEACAM1 antibody (<a href="/products/HA750658" style="font-weight: bold;text-decoration: underline;">HA750658</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750658" style="font-weight: bold;text-decoration: underline;">HA750658</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CEACAM1 antibody (HA750658) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750658) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CEACAM1 antibody (<a href="/products/HA750658" style="font-weight: bold;text-decoration: underline;">HA750658</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750658" style="font-weight: bold;text-decoration: underline;">HA750658</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CEACAM1 antibody (HA750658) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750658) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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metafield image

CEACAM1 Recombinant Rabbit Monoclonal Antibody [PSH0-78]

Application: WB,IHC-P

Reactivity: Human

Conjugate: unconjugated