CEACAM1 Recombinant Rabbit Monoclonal Antibody [PSH0-78]
Catalog# HA721507
CEACAM1 Recombinant Rabbit Monoclonal Antibody [PSH0-78]
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
CEACAM1 Recombinant Rabbit Monoclonal Antibody [PSH0-78]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CEACAM1 aa 35-428 / 526.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 58 kDa
Positive Control
SW480 cell lysate, A549 cell lysate, human kidney tissue, human liver tissue.
Conjugation
unconjugated
Clone Number
PSH0-78
RRID
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
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| Mouse |
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| Rat |
|
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
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1:1,000
Target
Function
Carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) (CEACAM1) also known as CD66a (Cluster of Differentiation 66a), is a human glycoprotein, and a member of the carcinoembryonic antigen (CEA) gene family. This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell–cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of only two has been determined. In melanocytic cells CEACAM1 gene expression may be regulated by MITF.
Background References
1. Park DJ et al. EpCAM-high liver cancer stem cells resist natural killer cell-mediated cytotoxicity by upregulating CEACAM1. J Immunother Cancer. 2020 Mar
2. To SKY et al. A Selective β-Catenin-Metadherin/CEACAM1-CCL3 Axis Mediates Metastatic Heterogeneity upon Tumor-Macrophage Interaction. Adv Sci (Weinh). 2022 May
Subcellular Location
Cell membrane, Lateral cell membrane, Apical cell membrane, Basal cell membrane, Cell junction, adherens junction.
UNIPROT
Synonyms
Antigen CD66 antibody
BGP 1 antibody
BGP antibody
BGP-1 antibody
BGPI antibody
Biliary glycoprotein 1 antibody
Biliary glycoprotein adhesion molecule antibody
Biliary glycoprotein antibody
Carcinoembryonic antigen related cell adhesion molecule 1 antibody
carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) antibody
ExpandImages
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Western blot analysis of CEACAM1 on different lysates with Rabbit anti-CEACAM1 antibody (HA721507) at 1/1,000 dilution and Mouse anti-6X His-tag antibody (HA600030) at 1/1,000 dilution.
Lane 1: His-tagged human CEACAM-1 recombinant protein aa (aa35-428) 20ng
Lane 2: His-tagged human CEACAM-5 recombinant protein aa (aa35-422) 20ng
Lane 3: His-tagged human CEACAM-6 recombinant protein aa (aa35-326) 20ng
Lane 4: His-tagged human CEACAM-8 recombinant protein aa (aa35-335) 20ng
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721507) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Western blot analysis of CEACAM1 on different lysates with Rabbit anti-CEACAM1 antibody (HA721507) at 1/1,000 dilution.
Lane 1: SW480 cell lysate (20 µg/Lane)
Lane 2: SW480 cell lysate (no heat) (20 µg/Lane)
Lane 3: A549 cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (no heat) (negative) (20 µg/Lane)
Lane 5: 293T cell lysate (negative) (20 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 58 kDa
Observed band size: 58-150 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721507) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CEACAM1 antibody (HA721507) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721507) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CEACAM1 antibody (HA721507) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721507) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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