Ubiquinol-Cytochrome C Reductase Core Protein I Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH01-23] (HA750699)
Catalog# HA750699
Rabbit Monoclonal to Ubiquinol-Cytochrome C Reductase Core Protein I
Tested for
-
WB
-
IHC-P
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IF-Tissue
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IF-Cell
Reacts with
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Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Ubiquinol-Cytochrome C Reductase Core Protein I on different lysates with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA750699) at 1/5,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: HCT 116 cell lysate (20 µg/Lane)
Lane 4: 293T cell lysate (20 µg/Lane)
Lane 5: PC-3M cell lysate (20 µg/Lane)
Lane 6: Jurkat cell lysate (20 µg/Lane)
Lane 7: NIH/3T3 cell lysate (20 µg/Lane)
Lane 8: PC-12 cell lysate (20 µg/Lane)
Lane 9: Mouse kidney tissue lysate (40 µg/Lane)
Lane 10: Mouse brain tissue lysate (40 µg/Lane)
Lane 11: Rat kidney tissue lysate (40 µg/Lane)
Lane 12: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 53 kDa
Observed band size: 45 kDa
Exposure time: 8 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750699) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: HCT 116 cell lysate (20 µg/Lane)
Lane 4: 293T cell lysate (20 µg/Lane)
Lane 5: PC-3M cell lysate (20 µg/Lane)
Lane 6: Jurkat cell lysate (20 µg/Lane)
Lane 7: NIH/3T3 cell lysate (20 µg/Lane)
Lane 8: PC-12 cell lysate (20 µg/Lane)
Lane 9: Mouse kidney tissue lysate (40 µg/Lane)
Lane 10: Mouse brain tissue lysate (40 µg/Lane)
Lane 11: Rat kidney tissue lysate (40 µg/Lane)
Lane 12: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 53 kDa
Observed band size: 45 kDa
Exposure time: 8 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750699) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.