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Ubiquinol-Cytochrome C Reductase Core Protein I Recombinant Rabbit Monoclonal Antibody [PSH01-23]

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Specification

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Catalog# HA721640

Ubiquinol-Cytochrome C Reductase Core Protein I Recombinant Rabbit Monoclonal Antibody [PSH01-23]

Application

  • WB

  • IHC-P

  • IF-Tissue

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Ubiquinol-Cytochrome C Reductase Core Protein I Recombinant Rabbit Monoclonal Antibody [PSH01-23]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human UQCRC1 aa 1-480 / 480.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue, IF-Cell

Molecular Weight

Predicted band size: 53 kDa

Positive Control

HeLa cell lysate, HepG2 cell lysate, HCT 116 cell lysate, 293T cell lysate, PC-3M cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse kidney tissue lysate, mouse brain tissue lysate, rat kidney tissue lysate, rat brain tissue lysate, human kidney tissue, human liver tissue, human small intestine tissue, mouse kidney tissue, HepG2, 293T.

Conjugation

unconjugated

Clone Number

PSH01-23

RRID

AB_3072753

Reactivity Data

WB IHC-P IF-Tissue IF-Cell
Human
Tested Tested
Tested Tested
Tested Tested
Tested Tested
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:5,000

  • IF-Tissue

  • 1:1,000

  • IF-Cell

  • 1:100

Target

Function

Cytochrome b-c1 complex subunit 1, mitochondrial is a protein that in humans is encoded by the UQCRC1 gene. Its gene product is a subunit of the respiratory chain protein Ubiquinol Cytochrome c Reductase (UQCR, Complex III or Cytochrome bc1 complex), which consists of the products of one mitochondrially encoded gene, MTCYTB (mitochondrial cytochrome b) and ten nuclear genes: UQCRC1, UQCRC2, Cytochrome c1, UQCRFS1 (Rieske protein), UQCRB, "11kDa protein", UQCRH (cyt c1 Hinge protein), Rieske Protein presequence, "cyt. c1 associated protein", and Rieske-associated protein.

Background References

1. Lin CH et al. Mitochondrial UQCRC1 mutations cause autosomal dominant parkinsonism with polyneuropathy. Brain. 2020 Dec

2. Hung YC et al. UQCRC1 engages cytochrome c for neuronal apoptotic cell death. Cell Rep. 2021 Sep

Subcellular Location

Mitochondrion inner membrane.

UNIPROT

SwissProt: P31930 Human

SwissProt: Q9CZ13 Mouse

SwissProt: Q68FY0 Rat

Synonyms

Complex III subunit 1 antibody

Core protein I antibody

Cytochrome b c1 complex subunit 1, mitochondrial antibody

Cytochrome b-c1 complex subunit 1 antibody

D3S3191 antibody

mitochondrial antibody

OTTHUMP00000164820 antibody

QCR1 antibody

QCR1_HUMAN antibody

Ubiquinol cytochrome c reductase core protein I antibody

Expand

Images

  • Western blot analysis of Ubiquinol-Cytochrome C Reductase Core Protein I on different lysates with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (<a href="/products/HA721640" style="font-weight: bold;text-decoration: underline;">HA721640</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: HepG2 cell lysate (20 µg/Lane)<br />Lane 3: HCT 116 cell lysate (20 µg/Lane)<br />Lane 4: 293T cell lysate (20 µg/Lane)<br />Lane 5: PC-3M cell lysate (20 µg/Lane)<br />Lane 6: Jurkat cell lysate (20 µg/Lane)<br />Lane 7: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 8: PC-12 cell lysate (20 µg/Lane)<br />Lane 9: Mouse kidney tissue lysate (40 µg/Lane)<br />Lane 10: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 11: Rat kidney tissue lysate (40 µg/Lane)<br />Lane 12: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 53 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 8 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721640" style="font-weight: bold;text-decoration: underline;">HA721640</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Ubiquinol-Cytochrome C Reductase Core Protein I on different lysates with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA721640) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: HepG2 cell lysate (20 µg/Lane)
    Lane 3: HCT 116 cell lysate (20 µg/Lane)
    Lane 4: 293T cell lysate (20 µg/Lane)
    Lane 5: PC-3M cell lysate (20 µg/Lane)
    Lane 6: Jurkat cell lysate (20 µg/Lane)
    Lane 7: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 8: PC-12 cell lysate (20 µg/Lane)
    Lane 9: Mouse kidney tissue lysate (40 µg/Lane)
    Lane 10: Mouse brain tissue lysate (40 µg/Lane)
    Lane 11: Rat kidney tissue lysate (40 µg/Lane)
    Lane 12: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 53 kDa
    Observed band size: 45 kDa

    Exposure time: 8 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721640) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (<a href="/products/HA721640" style="font-weight: bold;text-decoration: underline;">HA721640</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721640" style="font-weight: bold;text-decoration: underline;">HA721640</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA721640) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721640) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (<a href="/products/HA721640" style="font-weight: bold;text-decoration: underline;">HA721640</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721640" style="font-weight: bold;text-decoration: underline;">HA721640</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA721640) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721640) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (<a href="/products/HA721640" style="font-weight: bold;text-decoration: underline;">HA721640</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721640" style="font-weight: bold;text-decoration: underline;">HA721640</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA721640) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721640) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (<a href="/products/HA721640" style="font-weight: bold;text-decoration: underline;">HA721640</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721640" style="font-weight: bold;text-decoration: underline;">HA721640</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA721640) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721640) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Ubiquinol-Cytochrome C Reductase Core Protein I with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (<a href="/products/HA721640" style="font-weight: bold;text-decoration: underline;">HA721640</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA721640" style="font-weight: bold;text-decoration: underline;">HA721640</a>, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Ubiquinol-Cytochrome C Reductase Core Protein I with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA721640) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721640, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Ubiquinol-Cytochrome C Reductase Core Protein I with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (<a href="/products/HA721640" style="font-weight: bold;text-decoration: underline;">HA721640</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA721640" style="font-weight: bold;text-decoration: underline;">HA721640</a>, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Ubiquinol-Cytochrome C Reductase Core Protein I with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA721640) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721640, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunocytochemistry analysis of HepG2 cells labeling Ubiquinol-Cytochrome C Reductase Core Protein I with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (<a href="/products/HA721640" style="font-weight: bold;text-decoration: underline;">HA721640</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (<a href="/products/HA721640" style="font-weight: bold;text-decoration: underline;">HA721640</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HepG2 cells labeling Ubiquinol-Cytochrome C Reductase Core Protein I with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA721640) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA721640) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunocytochemistry analysis of 293T cells labeling Ubiquinol-Cytochrome C Reductase Core Protein I with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (<a href="/products/HA721640" style="font-weight: bold;text-decoration: underline;">HA721640</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (<a href="/products/HA721640" style="font-weight: bold;text-decoration: underline;">HA721640</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of 293T cells labeling Ubiquinol-Cytochrome C Reductase Core Protein I with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA721640) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA721640) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

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